Version 1
: Received: 9 July 2024 / Approved: 9 July 2024 / Online: 11 July 2024 (12:18:12 CEST)
How to cite:
Green, A. L.; Cowell, E. C.; Carr, L. M.; Hemsley, K.; Sherratt, E.; Collins-Praino, L.; Carr, J. M. Application of diceCT to Study the Development of the Zika Virus Infected Mouse Brain. Preprints2024, 2024070791. https://doi.org/10.20944/preprints202407.0791.v1
Green, A. L.; Cowell, E. C.; Carr, L. M.; Hemsley, K.; Sherratt, E.; Collins-Praino, L.; Carr, J. M. Application of diceCT to Study the Development of the Zika Virus Infected Mouse Brain. Preprints 2024, 2024070791. https://doi.org/10.20944/preprints202407.0791.v1
Green, A. L.; Cowell, E. C.; Carr, L. M.; Hemsley, K.; Sherratt, E.; Collins-Praino, L.; Carr, J. M. Application of diceCT to Study the Development of the Zika Virus Infected Mouse Brain. Preprints2024, 2024070791. https://doi.org/10.20944/preprints202407.0791.v1
APA Style
Green, A. L., Cowell, E. C., Carr, L. M., Hemsley, K., Sherratt, E., Collins-Praino, L., & Carr, J. M. (2024). Application of diceCT to Study the Development of the Zika Virus Infected Mouse Brain. Preprints. https://doi.org/10.20944/preprints202407.0791.v1
Chicago/Turabian Style
Green, A. L., Lyndsey Collins-Praino and Jillian M Carr. 2024 "Application of diceCT to Study the Development of the Zika Virus Infected Mouse Brain" Preprints. https://doi.org/10.20944/preprints202407.0791.v1
Abstract
Zika virus (ZIKV) impacts the developing brain. Here a technique was applied to define, in 3D, developmental changes in the brain of ZIKV-infected mice.
Postnatal day 1 mice were uninfected or ZIKV-infected, then analysed by iodine staining and micro-CT scanning (diffusible iodine contrast enhanced micro-CT; diceCT) at 3-, 6- and 10-days post infection (dpi). Multiple brain regions were visualised using diceCT; olfactory bulb, cerebrum, hippocampus, midbrain, interbrain, and cerebellum, along with the lens and retina of the eye. Brain regions were computationally segmented and quantitated, with increased brain volumes with developmental time in uninfected mice. Conversely, in ZIKV-infected mice, no quantitative differences were seen at 3 or 6 dpi, when there are no clinical signs, but qualitatively, diverse visual defects were identified at 6-10 dpi. By 10 dpi, ZIKV-infected mice had significantly lower body weight and reduced volume of brain regions compared to 10 dpi-uninfected or 6dpi ZIKV-infected mice. Nissl and immunofluorescent Iba1 staining on post-diceCT tissue were successful, but RNA extraction was not.
Thus, diceCT shows utility for detecting both 3D qualitative and quantitative changes in the developing brain of ZIKV-infected mice, with the benefit, post-diceCT, of retaining the ability to apply traditional histology and immunofluorescent analysis to tissue.
Keywords
CT; brain imaging; congenital zika syndrome
Subject
Biology and Life Sciences, Virology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
