Version 1
: Received: 15 July 2024 / Approved: 15 July 2024 / Online: 16 July 2024 (03:04:56 CEST)
How to cite:
Belal, M.; Ntini, C.; Sylvia, C.; Wassie, M.; Magdy, M.; Ogutu, C.; Ezzat, M.; Mollah, M. D. A.; Cao, Y.; Zhang, W.; Nishawy, E.; Han, Y. Genome-Wide Identification of AGO, DCL, and RDR Genes and Their Expression Analysis in Response to Drought Stress in Peach. Preprints2024, 2024071153. https://doi.org/10.20944/preprints202407.1153.v1
Belal, M.; Ntini, C.; Sylvia, C.; Wassie, M.; Magdy, M.; Ogutu, C.; Ezzat, M.; Mollah, M. D. A.; Cao, Y.; Zhang, W.; Nishawy, E.; Han, Y. Genome-Wide Identification of AGO, DCL, and RDR Genes and Their Expression Analysis in Response to Drought Stress in Peach. Preprints 2024, 2024071153. https://doi.org/10.20944/preprints202407.1153.v1
Belal, M.; Ntini, C.; Sylvia, C.; Wassie, M.; Magdy, M.; Ogutu, C.; Ezzat, M.; Mollah, M. D. A.; Cao, Y.; Zhang, W.; Nishawy, E.; Han, Y. Genome-Wide Identification of AGO, DCL, and RDR Genes and Their Expression Analysis in Response to Drought Stress in Peach. Preprints2024, 2024071153. https://doi.org/10.20944/preprints202407.1153.v1
APA Style
Belal, M., Ntini, C., Sylvia, C., Wassie, M., Magdy, M., Ogutu, C., Ezzat, M., Mollah, M. D. A., Cao, Y., Zhang, W., Nishawy, E., & Han, Y. (2024). Genome-Wide Identification of <em>AGO</em>, <em>DCL</em>, and <em>RDR</em> Genes and Their Expression Analysis in Response to Drought Stress in Peach. Preprints. https://doi.org/10.20944/preprints202407.1153.v1
Chicago/Turabian Style
Belal, M., Elsayed Nishawy and Yuepeng Han. 2024 "Genome-Wide Identification of <em>AGO</em>, <em>DCL</em>, and <em>RDR</em> Genes and Their Expression Analysis in Response to Drought Stress in Peach" Preprints. https://doi.org/10.20944/preprints202407.1153.v1
Abstract
To address the response of small RNAs (sRNAs) biogenesis to drought stress, we identified sRNAs biogenesis genes, including 11 encoding argonauts (AGO), 8 encoding dicer-like proteins (DCL), and 9 encoding RNA-dependent RNA polymerases (RDR) in the peach genome. Notably, the largest numbers of sRNAs biogenesis genes are located to chromosome 1. The PAZ, PIWI, and MID domains were identified in PpAGOs, while the ribonuclease IIIa and IIIb domains were characterized in PpDCLs. The RDRP domain was recognized in PpRDRs. Orthologous similarity and collinearity analyses between Arabidopsis and peach revealed 5, 1, and 2 collinear blocks in AGOs, DCLs, and RDRs, respectively. Moreover, 41, 40, and 42 cis-acting elements were located in the promoters of PpAGOs, PpDCLs, and PpRDRs, respectively, with the majority related to drought stress response. Analysis of RNA sequencing (RNA-seq) data revealed that sRNAs biogenesis genes were involved in drought stress response in different tissues. Furthermore, the expression of candidate genes was verified in two peach cultivars, Beijing 2-7 (BJ2-7) and Sinai (SN), which are drought tolerance and sensitive, respectively. Interestingly, the expression of PpAGO2b, PpDCL2b, PpDCL4, and PpRDR4 genes was induced in ‘BJ2-7’ but inhibited in ‘SN’ under drought stress. Overall, this study provides insight into the roles of sRNA biogenesis genes in response to drought stress in peach.
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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