Version 1
: Received: 16 July 2024 / Approved: 16 July 2024 / Online: 16 July 2024 (17:05:37 CEST)
How to cite:
Alieva, R. T.; Ulasov, A. V.; Khramtsov, Y. V.; Slastnikova, T. A.; Lupanova, T. N.; Gribova, M. A.; Georgiev, G. P.; Rosenkranz, A. A. Optimization of a Modular Nanotransporter Design for the Killing of Cancer Cells via Targeted Intracellular Delivery of a Photosensitizer. Preprints2024, 2024071330. https://doi.org/10.20944/preprints202407.1330.v1
Alieva, R. T.; Ulasov, A. V.; Khramtsov, Y. V.; Slastnikova, T. A.; Lupanova, T. N.; Gribova, M. A.; Georgiev, G. P.; Rosenkranz, A. A. Optimization of a Modular Nanotransporter Design for the Killing of Cancer Cells via Targeted Intracellular Delivery of a Photosensitizer. Preprints 2024, 2024071330. https://doi.org/10.20944/preprints202407.1330.v1
Alieva, R. T.; Ulasov, A. V.; Khramtsov, Y. V.; Slastnikova, T. A.; Lupanova, T. N.; Gribova, M. A.; Georgiev, G. P.; Rosenkranz, A. A. Optimization of a Modular Nanotransporter Design for the Killing of Cancer Cells via Targeted Intracellular Delivery of a Photosensitizer. Preprints2024, 2024071330. https://doi.org/10.20944/preprints202407.1330.v1
APA Style
Alieva, R. T., Ulasov, A. V., Khramtsov, Y. V., Slastnikova, T. A., Lupanova, T. N., Gribova, M. A., Georgiev, G. P., & Rosenkranz, A. A. (2024). Optimization of a Modular Nanotransporter Design for the Killing of Cancer Cells via Targeted Intracellular Delivery of a Photosensitizer. Preprints. https://doi.org/10.20944/preprints202407.1330.v1
Chicago/Turabian Style
Alieva, R. T., Georgii P. Georgiev and Andrey A. Rosenkranz. 2024 "Optimization of a Modular Nanotransporter Design for the Killing of Cancer Cells via Targeted Intracellular Delivery of a Photosensitizer" Preprints. https://doi.org/10.20944/preprints202407.1330.v1
Abstract
Modular nanotransporters (MNTs) are drug delivery systems for targeted cancer treatment. Being composed of several modules, they offer the advantage of high specificity and biocompatibility in delivering drugs to the target compartment of cancer cells. The large carrier module brings together functioning MNT modules and serves as a platform for drug attachment. The development of smaller-sized MNTs via truncation of the carrier module looks advantageous in facilitating tissue penetration. In this study, two new MNTs with a truncated carrier module containing either an N-terminal (MNTN) or a C-terminal (MNTC) part were developed by genetic engineering. Both new MNTs demonstrated high affinity for target receptors, as revealed by fluorescently labeled ligand-competitive binding. The liposome leakage assay proved the endosomolytic activity of MNTs. Binding to the importin heterodimer of each truncated MNT was revealed by a thermophoresis assay, while only MNTN possessed binding to Keap1. Finally, the photodynamic efficacy of the photosensitizer attached to MNTN was significantly higher than when attached to either MNTC or the original MNT. Thus, this work reveals that MNT’s carrier module can be truncated without losing MNT functionality, favoring the N-terminal part of the carrier module due to its ability to bind Keap1.
Medicine and Pharmacology, Medicine and Pharmacology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
