Version 1
: Received: 16 July 2024 / Approved: 17 July 2024 / Online: 17 July 2024 (08:11:47 CEST)
How to cite:
Chung, M.; Dudley, E.; Kittana, H.; Thompson, A. C.; Scott, M.; Norman, K.; Valeris-Chacin, R. Antimicrobial Resistance Genes in Clinical Salmonella Isolates From Cattle in the Texas Panhandle, USA. Preprints2024, 2024071371. https://doi.org/10.20944/preprints202407.1371.v1
Chung, M.; Dudley, E.; Kittana, H.; Thompson, A. C.; Scott, M.; Norman, K.; Valeris-Chacin, R. Antimicrobial Resistance Genes in Clinical Salmonella Isolates From Cattle in the Texas Panhandle, USA. Preprints 2024, 2024071371. https://doi.org/10.20944/preprints202407.1371.v1
Chung, M.; Dudley, E.; Kittana, H.; Thompson, A. C.; Scott, M.; Norman, K.; Valeris-Chacin, R. Antimicrobial Resistance Genes in Clinical Salmonella Isolates From Cattle in the Texas Panhandle, USA. Preprints2024, 2024071371. https://doi.org/10.20944/preprints202407.1371.v1
APA Style
Chung, M., Dudley, E., Kittana, H., Thompson, A. C., Scott, M., Norman, K., & Valeris-Chacin, R. (2024). Antimicrobial Resistance Genes in Clinical Salmonella Isolates From Cattle in the Texas Panhandle, USA. Preprints. https://doi.org/10.20944/preprints202407.1371.v1
Chicago/Turabian Style
Chung, M., Keri Norman and Robert Valeris-Chacin. 2024 "Antimicrobial Resistance Genes in Clinical Salmonella Isolates From Cattle in the Texas Panhandle, USA" Preprints. https://doi.org/10.20944/preprints202407.1371.v1
Abstract
Rising antimicrobial resistance (AMR) in Salmonella serotypes host-adapted to cattle is of increasing concern to the beef and dairy industry. The bulk of the existing literature focuses on AMR post-slaughter. In comparison, the understanding of AMR in Salmonella among pre-harvest cattle is still limited, particularly in Texas, which ranks top five in beef and dairy exports in the United States; inherently, the health of Texas cattle has nationwide implications for the health of the United States beef and dairy industry. In this study, long-read whole genome sequencing and bioinformatic methods were utilized to analyze antimicrobial resistance genes (ARGs) in 98 isolates from beef and dairy cattle in the Texas Panhandle. Fisher exact tests and elastic net models accounting for population structure were used to infer associations between ARGs and antimicrobial phenotypic profiles and metadata. Gene mapping was also performed to assess the role of mobile genetic elements in harboring ARGs. ARG were found to be statistically different between the type of cattle operation and Salmonella serotypes. Beef operations were statistically significantly associated with more resistance genes compared to dairy operations. Salmonella Heidelberg, followed by Salmonella Dublin isolates, were associated with the most ARGs. Additionally, specific classes of ARGs were only present within mobile genetic elements.
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
