PreprintArticleVersion 1This version is not peer-reviewed
The Effect of Carboxymethyl Cellulose on Gene Expression Related with Apoptosis, Cold Shock, Motility and Reactive Oxygen Species of Cryopreserved Dog Sperm
Version 1
: Received: 19 July 2024 / Approved: 19 July 2024 / Online: 19 July 2024 (10:53:25 CEST)
How to cite:
Ibrahim, S.; Jeon, Y.; Yu, I.-J. The Effect of Carboxymethyl Cellulose on Gene Expression Related with Apoptosis, Cold Shock, Motility and Reactive Oxygen Species of Cryopreserved Dog Sperm. Preprints2024, 2024071572. https://doi.org/10.20944/preprints202407.1572.v1
Ibrahim, S.; Jeon, Y.; Yu, I.-J. The Effect of Carboxymethyl Cellulose on Gene Expression Related with Apoptosis, Cold Shock, Motility and Reactive Oxygen Species of Cryopreserved Dog Sperm. Preprints 2024, 2024071572. https://doi.org/10.20944/preprints202407.1572.v1
Ibrahim, S.; Jeon, Y.; Yu, I.-J. The Effect of Carboxymethyl Cellulose on Gene Expression Related with Apoptosis, Cold Shock, Motility and Reactive Oxygen Species of Cryopreserved Dog Sperm. Preprints2024, 2024071572. https://doi.org/10.20944/preprints202407.1572.v1
APA Style
Ibrahim, S., Jeon, Y., & Yu, I. J. (2024). The Effect of Carboxymethyl Cellulose on Gene Expression Related with Apoptosis, Cold Shock, Motility and Reactive Oxygen Species of Cryopreserved Dog Sperm. Preprints. https://doi.org/10.20944/preprints202407.1572.v1
Chicago/Turabian Style
Ibrahim, S., Yubyeol Jeon and Il-Jeoung Yu. 2024 "The Effect of Carboxymethyl Cellulose on Gene Expression Related with Apoptosis, Cold Shock, Motility and Reactive Oxygen Species of Cryopreserved Dog Sperm" Preprints. https://doi.org/10.20944/preprints202407.1572.v1
Abstract
Carboxymethyl cellulose (CMC) can act as a nonpermeating cryoprotectant, reducing the toxici-ty of glycerol. The dog spermatozoa were diluted and frozen in freezing extender supplemented with 0 (control), 0.1, 0.25, 0.5, or 0.75 % (w/v) CMC. Post-thaw, the sperm kinematic parameters (CASA), viability (SYBR-14/PI), and acrosome integrity (FITC-PSA) were evaluated. The expres-sion of genes related to apoptosis (BCL2, BAX, and ANAXA), motility (BACTIN), cold shock proteins (YBX3), and ROS (CAT, ROMO1, and SMOX) was evaluated using qPCR. The total mo-tility was higher in the 0.1 % CMC group compared to the other groups (p
Keywords
Carboxymethyl cellulose; dog sperm; cryopreservation; gene expression
Subject
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
