Preprint Article Version 1 This version is not peer-reviewed

Epidemiology and Genetic Susceptibility of SARS-CoV-2 of Young Children Living in Northwestern Amazon Region Presenting Acute Gastroenteritis and Respiratory Infection

Yan Cardoso Pimenta
,
Flávia Freitas de Oliveira Bonfim
,
Carlos Eduardo da Silva Figueiredo
,
Bruno Loreto Aragão
,
Mauro França Silva
,
Alberto Ignácio Olivares Olivares
,
José Paulo Gagliardi Leite
,
Isabella Fernandes Delgado
,
Marcia Terezinha Baroni de Moraes
*
Version 1 : Received: 29 July 2024 / Approved: 30 July 2024 / Online: 31 July 2024 (07:48:15 CEST)

How to cite: Pimenta, Y. C.; Bonfim, F. F. D. O.; Figueiredo, C. E. D. S.; Aragão, B. L.; Silva, M. F.; Olivares, A. I. O.; Leite, J. P. G.; Delgado, I. F.; Moraes, M. T. B. D. Epidemiology and Genetic Susceptibility of SARS-CoV-2 of Young Children Living in Northwestern Amazon Region Presenting Acute Gastroenteritis and Respiratory Infection. Preprints 2024, 2024072398. https://doi.org/10.20944/preprints202407.2398.v1 Pimenta, Y. C.; Bonfim, F. F. D. O.; Figueiredo, C. E. D. S.; Aragão, B. L.; Silva, M. F.; Olivares, A. I. O.; Leite, J. P. G.; Delgado, I. F.; Moraes, M. T. B. D. Epidemiology and Genetic Susceptibility of SARS-CoV-2 of Young Children Living in Northwestern Amazon Region Presenting Acute Gastroenteritis and Respiratory Infection. Preprints 2024, 2024072398. https://doi.org/10.20944/preprints202407.2398.v1

Abstract

This study evaluated the epidemiological and genetic susceptibility profile caused by coronavirus 2 (SARS-CoV-2) in children ≤3 years of age with acute gastroenteritis (AGE) or acute respiratory infection (ARI), living in the northwestern region of the Amazon (NWAR). Fecal and saliva sam-ples collected from 202 children (n=404) were subjected to detection of SARS-CoV-2 by RT-qPCR. Polymorphisms in the angiotensin-converting enzyme (ACE rs4646994) and ACE2 G8780A; rs2285666) genes were detected by SYBR GREEN real-time PCR and PCR/Alul digestion, respec-tively. Secretory and Lewis status of histo-blood group antigen (HBGA) was performed using phenotyping/ELISA. Frequencies for the AGE group: SARS-CoV-2; 8.9%; ARI group: SARS-CoV-2; 2.9%. The ACE I/I homozygous polymorphism gene was majority. Males with the ACE2 G8780A SNP were more susceptible to ARI symptoms than AGE (OR = 3.85; 95% CI-1.42-10.38; P 0.007). Most children were secretory FUT2 phenotype (92.6%). Surveillance of diarrhea and respiratory infections in children, along with understanding their causes, are crucial for identifying preven-tion and control strategies and for prioritizing and developing new vaccines. Clinical manifesta-tions of COVID-19 in children ≤3 years of age include AGE.

Keywords

COVID-19; SARS-CoV-2; susceptibility; histo-blood group antigen; angiotensin-converting enzyme

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.