PreprintBrief ReportVersion 1This version is not peer-reviewed
IL-10 and TGF-β, but Not IL-17A or IFN-G, Potentiate the IL-15-Induced Expansion of Human T Cells: Association with the Expression of β2m-Free HLA Class I Molecules.
Version 1
: Received: 30 July 2024 / Approved: 31 July 2024 / Online: 2 August 2024 (09:35:26 CEST)
How to cite:
Duarte, L. H.; Peixoto, H. A.; Cardoso, E. M.; Esgalhado, A. J.; Arosa, F. A. IL-10 and TGF-β, but Not IL-17A or IFN-G, Potentiate the IL-15-Induced Expansion of Human T Cells: Association with the Expression of β2m-Free HLA Class I Molecules.. Preprints2024, 2024072547. https://doi.org/10.20944/preprints202407.2547.v1
Duarte, L. H.; Peixoto, H. A.; Cardoso, E. M.; Esgalhado, A. J.; Arosa, F. A. IL-10 and TGF-β, but Not IL-17A or IFN-G, Potentiate the IL-15-Induced Expansion of Human T Cells: Association with the Expression of β2m-Free HLA Class I Molecules.. Preprints 2024, 2024072547. https://doi.org/10.20944/preprints202407.2547.v1
Duarte, L. H.; Peixoto, H. A.; Cardoso, E. M.; Esgalhado, A. J.; Arosa, F. A. IL-10 and TGF-β, but Not IL-17A or IFN-G, Potentiate the IL-15-Induced Expansion of Human T Cells: Association with the Expression of β2m-Free HLA Class I Molecules.. Preprints2024, 2024072547. https://doi.org/10.20944/preprints202407.2547.v1
APA Style
Duarte, L. H., Peixoto, H. A., Cardoso, E. M., Esgalhado, A. J., & Arosa, F. A. (2024). IL-10 and TGF-β, but Not IL-17A or IFN-G, Potentiate the IL-15-Induced Expansion of Human T Cells: Association with the Expression of β2m-Free HLA Class I Molecules.. Preprints. https://doi.org/10.20944/preprints202407.2547.v1
Chicago/Turabian Style
Duarte, L. H., André J Esgalhado and Fernando A. Arosa. 2024 "IL-10 and TGF-β, but Not IL-17A or IFN-G, Potentiate the IL-15-Induced Expansion of Human T Cells: Association with the Expression of β2m-Free HLA Class I Molecules." Preprints. https://doi.org/10.20944/preprints202407.2547.v1
Abstract
Background: IL-15 is a homeostatic cytokine for human T and NK cells. However, whether other cytokines influence the effect of IL-15 is not known. We have studied the impact that IL-10, TGF-, IL-17A and IFN- have on the IL-15-induced proliferation of human T cells and the expression of HLA class I (HLA-I) molecules. Methods: Peripheral blood lymphocytes (PBL) were labeled with CFSE, and stimulated for 12 days with IL-15 in the absence or presence of the other cytokines. The proportion of proliferating T cells and the expression of cell surface HLA-I molecules were analyzed by flow cytometry. Results: The IL-15-induced proliferation and expansion of T cells was paralleled by an increase in the expression of HC-10-reactive HLA-I molecules, namely on T cells that underwent ≥5-6 cycles of cell division. Noteworthy, the IL-15-induced proliferation of T cells was potentiated by IL-10 and TGF-β, but not by IL-17 or IFN-, and was associated with a decrease in the expression of HC-10-reactive molecules. Conclusions: The cytokines IL-10 and TGF-β potentiate the proliferative capacity that IL-15 has on human T cells in vitro, an effect that is associated with a reduction in the amount of HC-10 reactive HLA class I molecules induced by IL-15.
Keywords
Cytokines; CD8+ T Cells; Proliferation; HLA class I molecules; Open conformers; W6/32; HC-10
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
