PreprintArticleVersion 1This version is not peer-reviewed
1,2-Dicinnamoyl-Sn-Glycero-3-Phosphocholine Enhances Insulin Sensitivity and Increases the Expression Level of mtDNA-Encoded Genes in Insulin-Resistant 3T3-L1 Adipocytes – a Preliminary Study
Version 1
: Received: 5 August 2024 / Approved: 5 August 2024 / Online: 5 August 2024 (13:46:24 CEST)
How to cite:
Cierzniak, A.; Gliszczyńska, A.; Małodobra-Mazur, M. 1,2-Dicinnamoyl-Sn-Glycero-3-Phosphocholine Enhances Insulin Sensitivity and Increases the Expression Level of mtDNA-Encoded Genes in Insulin-Resistant 3T3-L1 Adipocytes – a Preliminary Study. Preprints2024, 2024080342. https://doi.org/10.20944/preprints202408.0342.v1
Cierzniak, A.; Gliszczyńska, A.; Małodobra-Mazur, M. 1,2-Dicinnamoyl-Sn-Glycero-3-Phosphocholine Enhances Insulin Sensitivity and Increases the Expression Level of mtDNA-Encoded Genes in Insulin-Resistant 3T3-L1 Adipocytes – a Preliminary Study. Preprints 2024, 2024080342. https://doi.org/10.20944/preprints202408.0342.v1
Cierzniak, A.; Gliszczyńska, A.; Małodobra-Mazur, M. 1,2-Dicinnamoyl-Sn-Glycero-3-Phosphocholine Enhances Insulin Sensitivity and Increases the Expression Level of mtDNA-Encoded Genes in Insulin-Resistant 3T3-L1 Adipocytes – a Preliminary Study. Preprints2024, 2024080342. https://doi.org/10.20944/preprints202408.0342.v1
APA Style
Cierzniak, A., Gliszczyńska, A., & Małodobra-Mazur, M. (2024). 1,2-Dicinnamoyl-Sn-Glycero-3-Phosphocholine Enhances Insulin Sensitivity and Increases the Expression Level of mtDNA-Encoded Genes in Insulin-Resistant 3T3-L1 Adipocytes – a Preliminary Study. Preprints. https://doi.org/10.20944/preprints202408.0342.v1
Chicago/Turabian Style
Cierzniak, A., Anna Gliszczyńska and Małgorzata Małodobra-Mazur. 2024 "1,2-Dicinnamoyl-Sn-Glycero-3-Phosphocholine Enhances Insulin Sensitivity and Increases the Expression Level of mtDNA-Encoded Genes in Insulin-Resistant 3T3-L1 Adipocytes – a Preliminary Study" Preprints. https://doi.org/10.20944/preprints202408.0342.v1
Abstract
Insulin resistance is a condition characterized by a reduced biological response to insulin. It is one of the most common metabolic diseases of modern civilization. Numerous naturally occurring substances have shown to have a positive effect in restoring the proper sensitivity to insulin. Mature 3T3-L1 adipocytes with artificially induced insulin resistance by palmitic acid (16:0) were used for the study. Cinnamic acid and 1,2-dicinnamoyl-sn-glycero-3-phosphocholin (1,2-diCA-PC) were tested at three concentrations: 25 μM, 50 μM, and 125 μM. The number of mitochondria and the expression of genes encoded by mtDNA were elucidated in control and experimental cells. In experimental cells treated with 1,2-diCA-PC, we displayed increased insulin-stimulated glucose uptake in a dose-dependent manner, accompanied by an increase in mtDNA copy number. Moreover, in experimental cells treated with 1,2-diCA-PC at the concentration of 125 μM, we observed a significant increase in the expression level of all analyzed genes encoded by mtDNA compared to control cells. Our study showed a relationship between improved cellular sensitivity to insulin by 1,2-diCA-PC and an increase in the number of mitochondria and expression levels of genes encoded by mtDNA. Summarizing, the results suggest a therapeutic potential of cinnamic acid derivative 1,2-diCA-PC in enhancing the insulin sensitivity of adipocytes.
Medicine and Pharmacology, Medicine and Pharmacology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
