In Vitro Self-Circularization Methods Based on Self-Splicing Ribozyme

Kyung Hyun Lee; Nan-Ee Lee; Seong-Wook Lee

doi:10.20944/preprints202408.0377.v1

Preprint Review Version 1 This version is not peer-reviewed

Version 1 : Received: 5 August 2024 / Approved: 5 August 2024 / Online: 6 August 2024 (12:42:47 CEST)

In vitro circular RNA (circRNA) preparation methods have been gaining a lot of attention recently as several reports suggest that circRNAs are more stable with better performances in cells and in vivo than linear RNAs in various biomedical applications. Self-splicing ribozymes are considered a major in vitro circRNA generation method for biomedical applications due to their simplicity and efficiency in circularization of gene of interest. This review summarizes updates and discusses recently developed self-circularization methods based on the self-splicing ribozyme such as group I and II intron ribozymes and pros and cons of each method to prepare circRNA in vitro.

Circular RNA; Self-circularization; Self-splicing; RNA vaccine; RNA therapeutics; Ribozyme; Group I intron; Group II intron

Medicine and Pharmacology, Medicine and Pharmacology

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In Vitro Self-Circularization Methods Based on Self-Splicing Ribozyme

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