Mitomycin C as an Anti-persister Strategy Against Klebsiella Pneumoniae: Toxicity and Synergy Studies

How to cite: Pacios, O.; Herrera-Espejo, S.; Armán, L.; Ibarguren-Quiles, C.; Blasco, L.; Bleriot, I.; Fernández-García, L.; Ortiz-Cartagena, C.; Paniagua, M.; Barrio Pujante, A.; Aracil, B.; Cisneros, J. M.; Pachon, M. E.; Tomás, M. Mitomycin C as an Anti-persister Strategy Against Klebsiella Pneumoniae: Toxicity and Synergy Studies. Preprints 2024, 2024080398. https://doi.org/10.20944/preprints202408.0398.v1 Pacios, O.; Herrera-Espejo, S.; Armán, L.; Ibarguren-Quiles, C.; Blasco, L.; Bleriot, I.; Fernández-García, L.; Ortiz-Cartagena, C.; Paniagua, M.; Barrio Pujante, A.; Aracil, B.; Cisneros, J. M.; Pachon, M. E.; Tomás, M. Mitomycin C as an Anti-persister Strategy Against Klebsiella Pneumoniae: Toxicity and Synergy Studies. Preprints 2024, 2024080398. https://doi.org/10.20944/preprints202408.0398.v1

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MDPI and ACS Style

Pacios, O.; Herrera-Espejo, S.; Armán, L.; Ibarguren-Quiles, C.; Blasco, L.; Bleriot, I.; Fernández-García, L.; Ortiz-Cartagena, C.; Paniagua, M.; Barrio Pujante, A.; Aracil, B.; Cisneros, J. M.; Pachon, M. E.; Tomás, M. Mitomycin C as an Anti-persister Strategy Against Klebsiella Pneumoniae: Toxicity and Synergy Studies. Preprints 2024, 2024080398. https://doi.org/10.20944/preprints202408.0398.v1

APA Style

Pacios, O., Herrera-Espejo, S., Armán, L., Ibarguren-Quiles, C., Blasco, L., Bleriot, I., Fernández-García, L., Ortiz-Cartagena, C., Paniagua, M., Barrio Pujante, A., Aracil, B., Cisneros, J. M., Pachon, M. E., & Tomás, M. (2024). Mitomycin C as an Anti-persister Strategy Against Klebsiella Pneumoniae: Toxicity and Synergy Studies. Preprints. https://doi.org/10.20944/preprints202408.0398.v1

Chicago/Turabian Style

Pacios, O., Maria Eugenia Pachon and María Tomás. 2024 "Mitomycin C as an Anti-persister Strategy Against Klebsiella Pneumoniae: Toxicity and Synergy Studies" Preprints. https://doi.org/10.20944/preprints202408.0398.v1

Abstract

The combination of several therapeutic strategies is often seen as a good way to decrease the resistance rates, since bacteria overcome better single-drug treatments than multi-drug ones. This strategy is especially attractive when several targets and subpopulations are affected, as it is the case of Klebsiella pneumoniae persister cells, a subpopulation of bacteria able to transiently survive antibiotic exposures. This work aims to evaluate the potential of a repurposed anticancer drug, mitomycin C, combined with the K. pneumoniae lytic phage vB_KpnM-VAC13 in vitro and the safety in an in vivo murine model against two clinical isolates of this pathogen, one of them exhibiting an imipenem-persister phenotype. At the same time, we verified the absence of toxicity of mitomycin C at the concentration using the human chondrocyte cell line T/C28a2. The viability of these human cells was checked both by cytotoxicity assays and flow cytometry.

Keywords

persistence; K. pneumoniae; repurposing; mitomycin C; lytic phage; cytotoxicity

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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