Version 1
: Received: 8 August 2024 / Approved: 9 August 2024 / Online: 9 August 2024 (12:11:58 CEST)
How to cite:
Kim, Y. J.; CHANG, J. H. Lipase B Immobilized Silica-coated Magnetic Nanoparticles for Rapid Hydrolysis, Acylation, and Lipolysis. Preprints2024, 2024080671. https://doi.org/10.20944/preprints202408.0671.v1
Kim, Y. J.; CHANG, J. H. Lipase B Immobilized Silica-coated Magnetic Nanoparticles for Rapid Hydrolysis, Acylation, and Lipolysis. Preprints 2024, 2024080671. https://doi.org/10.20944/preprints202408.0671.v1
Kim, Y. J.; CHANG, J. H. Lipase B Immobilized Silica-coated Magnetic Nanoparticles for Rapid Hydrolysis, Acylation, and Lipolysis. Preprints2024, 2024080671. https://doi.org/10.20944/preprints202408.0671.v1
APA Style
Kim, Y. J., & CHANG, J. H. (2024). Lipase B Immobilized Silica-coated Magnetic Nanoparticles for Rapid Hydrolysis, Acylation, and Lipolysis. Preprints. https://doi.org/10.20944/preprints202408.0671.v1
Chicago/Turabian Style
Kim, Y. J. and JEONG HO CHANG. 2024 "Lipase B Immobilized Silica-coated Magnetic Nanoparticles for Rapid Hydrolysis, Acylation, and Lipolysis" Preprints. https://doi.org/10.20944/preprints202408.0671.v1
Abstract
This study reports rapid hydrolysis, acylation, and lipolysis with lipase B from Candida antarctica (CalB) and nanofructosome encapsulated CalB (CalB@NF) enzymes immobilized on silica-coated magnetic nanoparticles (Si-MNPs). Enzymatic hydrolysis was confirmed for conversion to p-nitrophenol (p-NP, λex=400 nm) from p-nitrophenyl butyrate (p-NPB, λex=270 nm), and various catalytic parameters such as Km, Vmax, and Kcat were calculated from Michaelis-Menten and Lineweaver-Burk plot. Efficiency of enzymatic hydrolysis was in the order of CalB > Si-MNPs@CalB > CalB@NF > Si-MNPs@CalB@NF, and reusability was much better with the enzyme immobilized on Si-MNPs than with the native enzyme. Enzymatic acylation was confirmed by the formation of benzyl benzoate (λex=229 nm) from benzoic anhydride (λex=240 nm), and efficiency exceeded 99% in all samples. Finally, enzymatic lipolysis was confirmed by the decomposition of olive oil to fatty acid such as oleic acid (λex=230 nm) using thin-layer chromatography (TLC), column chromatography, and proton nuclear magnetic resonance (1H -NMR). The lipases immobilized on Si-MNPs have the advantage of good thermal stability and reusability and are thus applicable to a wide range of industries.
Keywords
Lipase, Immobilization; Magnetic nanoparticles; Lipolysis; Acylation; Hydrolysis
Subject
Chemistry and Materials Science, Chemical Engineering
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
