Preprint Article Version 1 This version is not peer-reviewed

Optimisation and Standardisation of the Extraction Method of Balanites aegyptiaca Del. Seeds (Zygophyllaceae) Used to Formulate a Phytomedicine

Mohamed Bonewendé Belemlilga
* ,
Salfo Ouedraogo
,
Gilchrist Abdoul Laurent Boly
,
Do Harouna Dao
,
Jonas Tiami Coulibaly
,
Jean Claude Romaric Pingdwindé Ouedraogo
,
Souleymane Compaoré
,
Sidiki Traore
,
Moumouni Koala
,
Estelle Noëla Hoho Youl
,
Lazare Belemnaba
,
Félix Bondo Kini
,
Aristide Traore
,
Sylvin Ouedraogo
Version 1 : Received: 9 August 2024 / Approved: 12 August 2024 / Online: 12 August 2024 (09:37:41 CEST)

How to cite: Belemlilga, M. B.; Ouedraogo, S.; Boly, G. A. L.; Dao, D. H.; Coulibaly, J. T.; Ouedraogo, J. C. R. P.; Compaoré, S.; Traore, S.; Koala, M.; Youl, E. N. H.; Belemnaba, L.; Kini, F. B.; Traore, A.; Ouedraogo, S. Optimisation and Standardisation of the Extraction Method of Balanites aegyptiaca Del. Seeds (Zygophyllaceae) Used to Formulate a Phytomedicine. Preprints 2024, 2024080773. https://doi.org/10.20944/preprints202408.0773.v1 Belemlilga, M. B.; Ouedraogo, S.; Boly, G. A. L.; Dao, D. H.; Coulibaly, J. T.; Ouedraogo, J. C. R. P.; Compaoré, S.; Traore, S.; Koala, M.; Youl, E. N. H.; Belemnaba, L.; Kini, F. B.; Traore, A.; Ouedraogo, S. Optimisation and Standardisation of the Extraction Method of Balanites aegyptiaca Del. Seeds (Zygophyllaceae) Used to Formulate a Phytomedicine. Preprints 2024, 2024080773. https://doi.org/10.20944/preprints202408.0773.v1

Abstract

Balanites aegyptiaca del. (Zygophyllaceae) is widely used in traditional medicine, both human and veterinary, throughout Africa for its many properties, including antiparasitic properties. This experimental study aims to optimise the extraction conditions of the seeds of Balanites aegyptiaca del. Aqueous maceration was carried out with mass to volume ratios of 40%, 30%, 20%, 10% and 5% and extraction times of 6, 12, 24, 36 and 48 hours. Extraction yields, phytochemical screening, saponins assay, antioxidant activities (ABTS and FRAP) and antiparasitic tests on Heligmosomoides bakeri were used to compare the different extracts. The pharmaco-chemical study generally showed that aqueous maceration gave the best results, with a mass/volume ratio of 10% after 12 hours of maceration. The yield obtained was 28.03% with a saponins content of 13.81 mg/g. The antioxidant activities were 4.25 ± 0.17µg/mL by the ABTS method and 0.739µg/mL by the FRAP method. The larvicidal activity also showed that the 10%12h extract produced 100% larval mortality from 25ug/mL. These data provide a basis for guiding the choice of extraction process parameters in producing this antiparasitic phytomedicine.

Keywords

optimisation; Standardisation; Balanites aegyptiaca; Antiparasitic phytomedicine

Subject

Medicine and Pharmacology, Medicine and Pharmacology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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