Version 1
: Received: 9 August 2024 / Approved: 12 August 2024 / Online: 12 August 2024 (14:03:55 CEST)
How to cite:
Murcia, G.; Alonso, R.; Berli, F.; Arias, L.; Bianchimano, L.; Pontin, M.; Fontana, A.; Casal, J. J.; Piccoli, P. Quantitative Proteomics Analysis of ABA and GA3 Treated Malbec Berries Reveals Insights into H2O2 Scavenging and Anthocyanin Dynamics. Preprints2024, 2024080826. https://doi.org/10.20944/preprints202408.0826.v1
Murcia, G.; Alonso, R.; Berli, F.; Arias, L.; Bianchimano, L.; Pontin, M.; Fontana, A.; Casal, J. J.; Piccoli, P. Quantitative Proteomics Analysis of ABA and GA3 Treated Malbec Berries Reveals Insights into H2O2 Scavenging and Anthocyanin Dynamics. Preprints 2024, 2024080826. https://doi.org/10.20944/preprints202408.0826.v1
Murcia, G.; Alonso, R.; Berli, F.; Arias, L.; Bianchimano, L.; Pontin, M.; Fontana, A.; Casal, J. J.; Piccoli, P. Quantitative Proteomics Analysis of ABA and GA3 Treated Malbec Berries Reveals Insights into H2O2 Scavenging and Anthocyanin Dynamics. Preprints2024, 2024080826. https://doi.org/10.20944/preprints202408.0826.v1
APA Style
Murcia, G., Alonso, R., Berli, F., Arias, L., Bianchimano, L., Pontin, M., Fontana, A., Casal, J. J., & Piccoli, P. (2024). Quantitative Proteomics Analysis of ABA and GA3 Treated Malbec Berries Reveals Insights into H<sub>2</sub>O<sub>2</sub> Scavenging and Anthocyanin Dynamics. Preprints. https://doi.org/10.20944/preprints202408.0826.v1
Chicago/Turabian Style
Murcia, G., Jorge José Casal and Patricia Piccoli. 2024 "Quantitative Proteomics Analysis of ABA and GA3 Treated Malbec Berries Reveals Insights into H<sub>2</sub>O<sub>2</sub> Scavenging and Anthocyanin Dynamics" Preprints. https://doi.org/10.20944/preprints202408.0826.v1
Abstract
Abscisic acid (ABA) and gibberellic acid (GA3) are regulators of fruit color and sugar levels, and the application of these hormones is a common practice in commercial vineyards dedicated to the production of table grapes. However, the effects of exogenous ABA and GA3 on wine cultivars remains unclear. We investigated the impact of ABA and GA3 applications on Malbec grapevine berries across three developmental stages. We found similar patterns of berry total anthocyanin accumulation, induced by both treatments, closely associated with berry H2O2 levels. Quantitative proteomics revealed that ABA and GA3 positively modulated antioxidant defense proteins, mitigating H2O2. Consequently, proteins involved in phenylpropanoid biosynthesis were downregulated, leading to decreased anthocyanin content at almost ripe stage, particularly petunidin-3-G and peonidin-3-G. Additionally, we noted increased levels of the non-anthocyanins E-viniferin and quercetin in the treated berries, which may enhance H2O2 scavenging at almost ripe stage. Using a linear mixed-effects model, we found statistical significance of the fixed effects berry H2O2 and sugar contents, demonstrating their roles in anthocyanin accumulation. In conclusion, our findings suggest a common molecular mechanism by which ABA and GA3 influence berry H2O2 content, ultimately impacting anthocyanin dynamics during ripening.
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
