1. Introduction

2. Experimental

2.1. Materials and Methods

2.1.1. Materials

Aniline was obtained from Merck (Germany), sodium hydroxide was bought from Merck, (Republic of South Africa), Sodium sulfadiazine salt (sfz) and Vanadium(IV) oxide hydrate were purchased from Sigma-Aldrich (USA), Anhydrous zinc(II) chloride(ZnCl₂) and carbon(IV) sulfide (CS₂) were supplied by Associated Chemical Enterprises (Pty) Ltd (RSA). Deionised water was produced in house. All the chemicals were of analytical reagent grade and were used as bought, without further purification.

2.1.2. Instrumentations

Open-end capillary tube melting point determination was carried out on a STUART SMP11 melting point apparatus and recorded uncorrected. Molar conductivity of compound was recorded on a CRINSON EC- Meter BASIC 30+ conductivity meter. The UV-Vis spectra were measured using a Perkin-Elmer Lambda 25 UV-Vis Spectrometer. The FT-IR spectra were recorded from a KBr disc in the range of 370-4000 cm^-1 on a Perkin-Elmer 2000 FT-IR Spectrophotometer. The NMR spectra were recorded on a Varian Unity Inova 400 NMR spectrometer operating at frequencies of 400 MHz for ¹H NMR and at 150 MHz for ¹³C NMR frequencies. The ¹H NMR and ¹³C NMR were determined from solutions of the compounds in DMSO-d₆ as internal standard of TMS. The chemical shifts were in ppm in relation to internal standard of TMS. The crystals were collected, unit cell determined, and data collected from a four circles diffractometer Gemini of Oxford Diffraction, using a graphite monochromated CuKα radiation (λ = 1.54184 Å) before solving and refining them.

2.1.3. Methods

Compounds of (pl-dtc, [VO(sfz)(pl-dtc)] and [Zn(sfz)(pl-dtc)] were synthesized using the method of one pot synthesis [5].

• Sodium sulfadiazine (C₁₀H₉N₄NaO₂S; sfz)

It was used as bought as the first ligand between the mixed ligands.

White solid. Assay: ≥ 98%. M. P.> 300 ^oC. Molar Conductivity: 8.16 Ω^-1 cm² mol^-1, Selected FT-IR (KBr Pellets), v (cm^-1): 3404 (NH₂)_as 3268 (NH₂)_s; 3237 (SO₂NH); 1577 (C=N); 1229 (SO₂)_as; 1115 (SO₂)_s. Selected λ_max in DMSO solvent (nm): 274 (π-π^*, N-C=S);319 (π-π^*, S-C=S); 322, (n- π^*). ¹H NMR (DMSO-d₆, 400Hz, ppm): δ 8.10 (s, 1H, SO₂-NH₂); δ 7.47-7.48 (s, 1H, NH₂) δ 6.87-7.19 (m, 1H, C₆H₅-H) δ 5.40 (s, 1H, C=N).¹³C NMR (DMSO-d_6, 100.6 MHz, ppm) δ 164.19 (CSO₂-NH₂), δ 157.19 (¹³C-NH₂); δ 149.17 (N=¹³CH);δ 105.10-135.20 (C₆H₅-).

(i)

Synthesis of sodium phenyl dithiocarbamate (pl -dtc)

Aniline (9.11 mL, 0.10 mol), carbon(IV) sulfide (6.00 mL, 0.10mol) and sodium hydroxide (4.00 g, 0.10 mol) were used as starting materials. Whitish yellow crystals were obtained. Percentage Yield: 90%. M. P. 72 ^oC. Molar Conductivity: 1.09 Ω^-1 cm² mol^-1.Selected FT-IR (KBr Pellets), v (cm^-1): 3409 (NH₂)_as 3277 (NH₂)_s; 1517 (C-N), 981 (CS₂). Selected λ_max in DMSO solvent (nm): 246 (π-π^*, N-C=S); 317 (π-π^*, S-C=S); 381 (n- π^*). ¹H NMR (DMSO-d₆, 400Hz, ppm) δ 10.00 (s, 1H, NCS₂); δ 7.19-7.89(d, 2H NH₂);δ 6.87-7.16; 7.16-7.19 (d, t, m, Ar-H). ¹³C NMR (DMSO-d_6, 150 MHz, ppm) δ 214.93 (NCS₂), δ 143.76(s 1H NH₂), δ 137.23(s 1H HC-N), 125.68-128.85 (C₆H₅-H); The synthesis for pl-dtc is shown in Scheme 1.

Scheme 1. Synthesis of sodium phenyl dithiocarbamate (pl-dtc).

Scheme 1. Synthesis of sodium phenyl dithiocarbamate (pl-dtc).

(ii)

Synthesis of oxovanadium(IV) coordination compound of [VO(sfz)(pl-dtc)]

Vanadium (IV) oxide sulfate hydrate (0.25 g, 1.50 mmol), solution of sodium sulfadiazine, L₁, (0.41 g, 1.50 mmol) in methanol (100.00 mL) and aniline dithiocarbamate L_2pl-dtc, (0.29 g, 1.50 mmol) were magnetically stirred for 3 h at room temperature. A light green solid precipitate was formed, filtered, washed with deionized water (3 x 5 mL) and dried over silica gel in a desiccator. Percentage Yield: 75%. M. P. 250 ^oC. Molar Conductivity (DMSO): 0.74 Ω^-1 cm² mol^-1.Selected FT-IR (KBr Pellets), v (cm^-1): 3464 (NH₂)_as 3227 (NH₂)_s; 3150 (SO₂NH), 1615 (C=N), 1528 (C-N), 1223 (SO₂)_as; 1145 (SO₂)_s1002(CS₂) ; 934 (V=O), 395 (V-N) 466 (V-S). Selected λ_max in DMSO solvent (nm): 319 (π-π^*, N-C=S); 322 (π-π^*, S-C=S); 355 (n- π^*); Band I: 828, 738; Band II: 617; Band III: 396. The synthesis for oxovanadium(IV) coordination compound of [VO(sfz)(pl-dtc)] is shown in Equation 1.

Na-sfz_(aq) + pl-dtc_(l) + VOSO₄.xH₂O_(s)→ [VO(sfz)(pl-dtc)]_(s) + Na₂SO₄. xH₂O _(aq)…. (i)

(iii)

Synthesis of zinc(II) coordination compound of [Zn(sfz)(pl-dtc)]

Sodium sulfadiazine (0.4085 g, 1.5 mmol), sodium phenyl dithiocarbamate (L_2pl-dtc)(0.29 g, 1.50 mmol) and ZnCl₂ (0.20 g, 1.5 mmol) were used. A white solid was formed, washed and dried over silica gel. Yield: 72%. M. P. 216-218^oC. Molar Conductivity (DMSO): 0.01 Ω^-1 cm² mol^-1.Selected FT-IR (KBr), v(cm^-1): 3400 (NH₂)_as 3222 (NH₂)_s; 3090 (SO₂NH), 1518 (C=N), 1487(C-N), 1246 (SO₂)_as; 1141 (SO₂)_s . 963(CS₂)_as, 391 (Zn-N), 434 (Zn-S). Selected λ_max in DMSO solvent (nm): 235, 256 (π-π^*, N-C=S); 292 (π-π^*, S-C=S); 382, (n- π^*). Selected¹H NMR (DMSO-d₆, 400Hz, ppm): δ 10.08-10.11 (d 1H, H-NCS₂), δ 9.78; 8.47-8.50 (m, 1H, SO₂NH₂); δ 7.92 (-NH₂); δ 6.56-7.92 (d, t, Ar-H); δ 5.99 (s, 1H, C=H). Selected ¹³C NMR (DMSO-d_6, 150 MHz, ppm); δ 179.43 (NCS₂), δ 158.23(-SO₂N=C), 139.10 (NH₂C); δ112.14-128.44(Ar-C) δ. The synthesis for zinc(II) coordination compound of [Zn(sfz)(pl-dtc)] is shown in Equation (ii).

Na-sfz_(aq) + pl-dtc_(l) + ZnCl_2(s)→ [Zn(sfz)(pl-dtc)]_(s) + 2NaCl_(aq)…. (ii)

2.2. Crystal Growth and Crystallographic Activities

Crystals of sodium phenyl dithiocarbamate, (C₇H₁₂NNaO₃S₂) were grown out of solution when refrigerated after the fifth day. Crystals were washed with diethyl ether and subjected to single crystal x-ray crystallography. The crystals were collected and mounted on four circles diffractometer Gemini of Oxford Diffraction, using a graphite monochromated CuKα radiation (λ = 1.54184 Å). Super flip program was used to solve the crystal structure [12]. Refinement was done using full matrix least-squares technique with the support of F₂ with Jana 2006 [13]. Subsequent refinements were done with SHELXL-2018/3 [14] and the diagrams were drawn with ORTEP for Windows version 2020.1 [15]. All non-hydrogen atoms were refined anisotropically. Carbon-bound H atoms were placed in calculated positions and were included in the refinement in the riding model approximation, with U_iso(H) set to 1.2U_eq(C). The nitrogen and oxygen bound hydrogens were located on a difference map and allowed to refine freely. The crystal data, data collection, and refinements of sodium phenyl dithiocarbamate (pl-dtc) are shown in Table 1. Most bond lengths and angles are in good agreement with values have been deposited with the Cambridge Structural Database [16]

Table 1. Crystal data and details of the structure determination.

Table 1. Crystal data and details of the structure determination.

Crystal data
Formula	C7 H12 N Na O3 S2
Formula weight	245.29
Crystal system	orthorhombic
Space group	Pbcn (No. 60)
a (Å)	28.6599(5)
b (Å)	6.9376(1)
c (Å)	11.3162(2)
V (Å3)	2250.01(6)
Z	8
Dcalc (g cm-3)	1.448
μ(CuKa) (mm-1)	4.552
F(000)	1024
Crystal size (mm)	0.14 x 0.20 x 0.27
Crystal habit	Colourless polygon crystal
Temperature (K)	120
Radiation (Å)	CuKa 1.54184
Theta Min-Max (°)	3.1, 67.1
Dataset	-29:34; -8:8; -11:13
Tot. & unique data	12884, 2001
R(int)	0.031
Observed I > 2σ(I)	1852
Nref, Npar	2001, 156
R, wR2	0.0225, 0.0603
S	1.10
Min, Max residual density (e/Å3)	-0.18, 0.28
CCDC	2237964

3. Results and Discussion

3.1. Physicochemical Properties

The chemical reaction of mixed ligands of white solids of sfz and pl-dtc with hydrated blue oxovanadium(IV) ion yielded a coordination compound of light green solid precipitate of [VO(sfz)(pl-dtc)]. For synthesized zinc(II) of coordination compound of [Zn(sfz)(pl-dtc)], a white solid was formed compared to the mixed ligands of sfz and pl-dtc. Ligands were soluble in deionized water, dimethylformamide (DMF), dimethylsulfoxide (DMSO), and methanol, while the two coordination compounds were stable at room temperature, insoluble in deionized water, but soluble in DMF and DMSO solvents. Ligand of sfz and the two coordination compounds have higher melting points than pl-dtc due to their higher molecular masses than pl-dtc compound [17]. All compounds have molar conductivities less than 20 Ω^-1cm² mol^-1, which showed they are non electrolytic in nature [17]. The physicochemical parameters for all the compounds are shown in Table 2.

Table 2. Physicochemical parameters for studied compounds.

Table 2. Physicochemical parameters for studied compounds.

Compounds	Molecular formulae	Colour and state of matter	Assay/Yield (%)	Melting point (0°C)	Molar conductivity (Ω-1 cm2 mol-1)
Na-sfz	C10H9N4NaO2S	white solid	≥ 98	> 300	8.16
pl-dtc	C7H6NS2Na	whitish yellow crystals	90	72	1.09
[VO(sfz)(pl-dtc)]	C17H15N5S3O3Na2V	green solid	75	250	0.74
[Zn(sfz)(pl -dtc)]	C17H15N5S3O2Na2Zn	white solid	72	216-218	0.01

1.2. Infrared Spectroscopy

The changes before and after coordination of ligands to metal ions were observed in the two studied coordination compounds

• Coordination compound of [VO(sfz)(pl-dtc)]

Bellú et al. reported that the bands which appeared near 3500 and 3400 cm^-1 were due to asymmetric amino group (NH₂)_asy and symmetric amino group (NH₂)_sy [18]. The FT-IR of asymmetric amino group in sfz at 3404 cm^-1_(m) shifted slightly to lower frequency in the coordination compound of [VO(sfz)(pl-dtc)] appearing with a sharp peak at 3401cm^-1_(s). Similarly, the FT-IR of symmetric amino group has a frequency of 3268 cm^-1_(m) in sfz and also shifted to lower frequency in coordination compound of [(VOsfz)(pl-dtc)] at 3227 cm^-1_(sh). In line with Bellú et al, the FT-IR wavelengths are within 3500 and 3400 cm^-1 [18]. The slight differences between the ligand and the coordination compound indicated no involvement of the two amino groups in coordination [3,18].

For azomethine group (C=N), a medium band at 1584 cm^-1_(m) appeared in sfz, but shifted to higher and sharper frequency at 1615 cm^-1_(s) in [VO(sfz)(pl-dtc)]. This indicates a difference of 31 cm^-1 compared with sfz. The shift to higher frequency is in support of Athar et al.’s study which confirmed the presence of the azomethine band in the coordination [19]. Medium bands at 1229 cm^-1_(m) and 1115 cm^-1_(m) were assigned to the assymmetric and symmetric v(SO₂) in sfz respectively. Similarly, in [VO(sfz)(pl-dtc)] there are slight changes in SO₂ at 1223 cm^-1_(m) and 1145 cm^-1_(m) which were assigned to the assymmetric and symmetric v(SO₂)respectively [18,19]. The results due to slight difference agree with Bellú et al and Athar et al study that the sulfonamide oxygen (SO₂NH) did not participate in the coordination to metal ion [18,19]. The stretching vibration of the terminal V=O bond in [VO(sfz)(pl-dtc)] appeared with a sharp signal at 937 cm^-1_(shp)[20], while the V-N band has a frequency of 395cm^-1_(m) [20].

According to Odularu and Ajibade, infrared spectra of coordination compounds of dithiocarbamates have three distinct regions, which are region of 1580-1450 cm^-1 for the thioureide band ν(C-N), band^, region of 1060-940 cm^-1 for the ν(C=S), and region of 430-250 cm^-1 for the ν(M-S) [1]. Ligand of pl-dtc stretching vibration for ν(C-N) appeared sharply at a frequency of 1453 cm^-1, while shifted to higher frequency in [VO(sfz)(pl-dtc)] with a value of 65 cm^-1 to obtain 1528 cm^-1. The presence of the thioureide bond between double bond of ν(C=N) and single bond of ν(C-N) indicates partial delocalization of the π electron density of the thioureide bond [21]. Similarly, the ν(C=S) for pl-dtc appeared with a stretching vibration at 985 cm^-1 which shifted to higher frequency in [VO(sfz)(pl-dtc)] with a difference of 17 cm^-1 medium value of 1002 cm^-1.The ν(V-S) wavenumber is 466 cm^-1.

• Coordination compound of [Zn(sfz)(pl-dtc)]

The FT-IR of asymmetric amino group in sfz still at 3404 cm^-1_(m) shifted slightly to lower frequency in the coordination compound of [Zn(sfz)(pl-dtc)] appearing with a medium peak at 3401cm^-1_(s). In the same way, the FT-IR of symmetric amino group has a frequency of 3277 cm^-1_(m) in sfz and also shifted to lower frequency in coordination compound of [Zn(sfz)(pl-dtc)] at 3266 cm^-1_(sh). Bellú et al reported that the FT-IR wavelengths were within 3500 and 3400 cm^-1 [18]. The slight differences between the ligand and the coordination compound indicated no involvement of the two amino groups in coordination [3,18].

For azomethine group (C=N), a medium band at 1583 cm^-1_(m) appeared in sfz, but shifted with a difference of 5 cm^-1 to lower and medium frequency at 1578 cm^-1_(s) in [Zn(sfz)(pl-dtc)]. This indicates a difference of 5 cm^-1 compared with sfz. The shift to higher frequency is in support of Athar et al. study and confirmed the presence of the azomethine band in the coordination [19]. Medium bands at 1225 cm^-1_(m) and 1112 cm^-1_(m) are assigned to the assymmetric and symmetric v(SO₂)respectively in Na-sfz but in [Zn(sfz)(pl-dtc)] there are slight changes of 1246 cm^-1_(m) and 1141 cm^-1_(m) which could be assigned to the assymmetric and symmetric v(SO₂) respectively [18,19]. The results due to slight difference agree with Bellú et al. and Athar et al. that the sulfonamide oxygen (SO₂NH) did not participate in the coordination to metal ion [18,19]. The Zn-N band has a frequency of 391cm^-1_(shd) [20].

Ligand of pl-dtc stretching vibration for ν(C-N) appeared sharply at a frequency of 1517 cm^-1, while it shifted to lower frequency in [Zn(sfz)(pl-dtc)] with a difference of 30 cm^-1 to give a medium value of 1487 cm^-1. Similarly, the ν(C=S) for pl-dtc appeared with a stretching vibration at 981 cm^-1 and also shifted to lower frequency in [Zn(sfz)(pl-dtc)] with a difference of 18 cm^-1 to give a small value of 963 cm^-1. The ν(Zn-S) wavenumber is 551 cm^-1.

The FT-IR results showed that the coordination modes of sfz and pl-dtc litigated to oxidovanadium(IV) and zinc(II) ions respectively. The FTIR results for all studied compounds are shown in Table 3.

Table 3. The FT-IR results for all studied compounds.

Table 3. The FT-IR results for all studied compounds.

	ν(N-H)asy	ν(N-H)sy	ν(C=N)	ν(SO2)asy	ν(SO2)sy	ν(C-N)	ν(CS2)	ν(V=O)	ν(M-N)	ν(M-S)
sfz	3404(m)	3268 (m)	1577(m)	1227(m)	1115(m)	1453(shp)	985 (sm)
pl-dtc	3409(m)	3277(m)		1225(m)	1112(m)	1517(sm)	981(sm)
[VO(sfz)(pl-dtc)]	3401(shp)	3227(shd)	1615(m)	1223(m)	1145(m)	1528(sm)	1002(sm)	937(shp)	395(m)	466(m)
[Zn(sfz)(pl-dtc)]	3400(m)	3266(sm)	1578(m)	1246(sm)	1141(shp)	1487(m)	963(sm)		391(shd)	551(m)

Key: sfz: sodium sulfadiazine, pl-dtc (ai-dtc): sodium salt of phenyl dithiocarbamate, [VO(sfz)(pl-dtc)/(ai-dtc)]: coordination compound of oxidovanadium(IV) ion, [Zn(sfz)(pl-dtc)/(ai-dtc)]: coordination compound of oxovanadium(IV) ion, m: medium, shd: shoulder, shp: sharp, sm: small.

Figure 2. Merged spectra of Na-sfz, pl-dtc (ai-dtc) and [VO(sfz)(pl-dtc)/(ai-dtc)].

Figure 2. Merged spectra of Na-sfz, pl-dtc (ai-dtc) and [VO(sfz)(pl-dtc)/(ai-dtc)].

Figure 3. Merged spectra of Na-sfz, pl-dtc (ai-dtc), and [Zn(sfz)(pl-dtc)/(ai-dtc)].

Figure 3. Merged spectra of Na-sfz, pl-dtc (ai-dtc), and [Zn(sfz)(pl-dtc)/(ai-dtc)].

3.3. Electronic Spectroscopy

The electronic spectra for sfz, pl-dtc, [VO(sfz)(pl-dtc)] [Zn(sfz)(pl-dtc)] were recorded in the ultraviolet-visible range between 200 and 900 nm in 10^-3 solution of DMSO solvent. The coordination processes of sfz and pl-dtc and their corresponding coordination compounds were assessed from their electronic spectra for coordination activities and geometries. Both ligands share similar electronic spectra’ regions to each other. In the ultraviolet-visible region, dithiocarbamates generally show three bands related to intramolecular charge transfer [22]. These bands are π-π^* which corresponds to N-C=S, π-π^* which corresponds to S-C=S and n-π^* [22]. Another transition known as d-d transition was observed in the coordination compound of [VO (sfz)(pl-dtc)], which was due to excitation of the metal ions [22]. The chromophores of N-C=S and S-C=S were present in both ligands. Coordination compound of oxovanadium(IV) was red shifted with respect to Na-sfz and pl-dtc. The weak d-d transitions consist of Band I (828, 738 nm), Band II (617 nm) and Band III (396 nm) [20,23]. The red shift was due to the octahedral geometry of the coordination compound in line with Doadrio et al’s results [20]. In the case of [Zn(sfz)(pl-dtc)], it was blue shifted when compared with sfz and pl-dtc. The diamagnetic character of [Zn(sfz)(pl-dtc)] made it to be assumed to have tetrahedral geometry [24]. Results obtained from electronic spectra are shown in Table 4.

Table 4. Results obtained from electronic spectra.

Table 4. Results obtained from electronic spectra.

	π-π* (nm)		n-π* (nm)	d-d transitions (nm)
	N-C=S	S-C=S
sfz	274	319	322
pl-dtc	246	317	381
				Band I	Band II	Band III
[VO (sfz)(pl-dtc)]	319	322	355	828 738	617	396
[Zn(sfz)(pl-dtc)]	235 256	292	382

3.4. Nuclear Magnetic Resonance Spectroscopy (NMR)

This study used both proton NMR (¹H NMR) and carbon 13 NMR (¹³C NMR) for structural determination of Na-sfz, pl-dtc and [Zn(sfz)(pl-dtc)]. The paramagnetic nature of [VO(sfz)(pl-dtc)] did not allow the use of ¹H NMR and ¹³C NMR .

• Proton NMR (¹H NMR)

The ¹H NMR spectra for sfz, pl-dtc, and [Zn(sfz)(pl-dtc)] were recorded at room temperature. Deuterated dimethyl sulfoxide (dmso-d₆) was used as the internal reference. The results obtained were in agreement with to literature [22]. The proton in sulfonamide nitrogen (SO₂-NH₂) of sfz appeared as singlet and has a signal at δ 8.10 ppm (Figure 4), which moved slightly downfield to δ 9.78, 8.50 ppm with doublet in [Zn(sfz)(pl-dtc)] (Figure 6) [25]. This indicates it was not involved in the coordination. The amino group of the sfz, which also appeared singlet at δ 7.47-7.48 ppm also deshielded slightly to δ 7.91 ppm as singlet in [Zn(sfz)(pl-dtc)] [25,26]. The amino groups are, as well not involved in the coordination. The aromatic protons appeared as doublet in sfz because of the presence of the two aromatic rings at δ 6.50 ppm and shifted downfield slightly to δ 6.60 ppm in [Zn(sfz)(pl-dtc)] [25]. The azomethine proton signaled as a singlet in sfz at δ 5.40 ppm and shifted downfield remaining singlet at δ 5.99 ppm in [Zn(sfz)(pl-dtc)] [26].

In the pl-dtc (Figure 5), the amino group of pl-dtc, appeared doublet at δ 7.19-7.89 ppm also deshielded slightly to δ 7.91 ppm (overlapping) also as doublet in [Zn(sfz)(pl-dtc)]. The dithiocarbamato proton (-HNCS₂) as singlet at δ 10.00 ppm shifted downfield slightly to δ 10.11 ppm. The aromatic protons resonated as doublet and triplet at δ 6.87-7.16 and δ 7.16-7.19 and δ 7.19-7.89 ppm and became deshielded with multiple singlets at δ 6.56- 7.62 ppm in [Zn(sfz)(pl-dtc)] [27,28].

Figure 4. The ¹H NMR of sfz.

Figure 4. The ¹H NMR of sfz.

Figure 5. The ¹H NMR of pl-dtc.

Figure 5. The ¹H NMR of pl-dtc.

Figure 6. The ¹H NMR of [Zn(sfz)(pl-dtc)].

Figure 6. The ¹H NMR of [Zn(sfz)(pl-dtc)].

• Carbon 13 NMR (¹³C NMR) and hybridization

For the hybridization of the carbon atoms, sp³ hybridized carbon atoms resonate from 0 to 90 ppm, while sp² hybridized carbon atoms resonate from 110 to 220 ppm. All the studied compounds possess both sp³ and sp² hybridized carbon atoms, therefore, resonance occurred from 0 to 220 ppm. Differences in chemical shifts between ligands and coordination compounds indicate a ligation between ligands and metal ions [29].

Carbon of the of aromatic rings resonated in sfz (Figure 7) at δ105.10, δ 110.91, δ 120.25, δ 135.20 ppm coincided with the carbons in the aromatic rings of pl-dtc (Figure 8) δ (125.68, 125. 79, 127.94, 128. 85 and 137.23 ppm) for both to have signals which appeared at δ (112.14, δ 121.87, δ 123.74, δ 128.55, δ 128. 44 and δ 139.10 ppm) in [Zn(sfz)(pl-dtc)] (Figure 9) [26]. Carbons of the azomethine group and amino group resonated at δ 149.17 and δ 157.19 ppm respectively, but shifted downfield to δ 158.23 ppm by overlapping in [Zn(sfz)(pl-dtc)] [25].

The carbon of dithiocarbamato moiety resonated at δ 164. 19 ppm and became downshielded to 179.43 ppm in [Zn(sfz)(pl-dtc)] [28,29]. On the other hand, the thioureide carbon in pl-dtc resonated at 143.76 ppm and was downfielded to 158. 23 ppm in [Zn(sfz)(pl-dtc)] [28,29]. Dithiocarbamato moiety, (N¹³CS₂) in pl-dtc resonated at δ 214.93 ppm and had a upfield shifting at δ 179.43 ppm in [Zn(SFZ)(pl-dtc)] [1,28,29,30].

Figure 7. The ¹³C NMR of sfz.

Figure 7. The ¹³C NMR of sfz.

Figure 8. The ¹³C NMR of pl-dtc.

Figure 8. The ¹³C NMR of pl-dtc.

Figure 9. The ¹³C NMR of [Zn(sfz)(pl-dtc).

Figure 9. The ¹³C NMR of [Zn(sfz)(pl-dtc).

3.5. Crystal Data, Data Collection and Refinements of pl-dtc

The crystal and packing structures of are shown in Figure 10 and Figure 11 respectively. The structure consists of a sodium atom with an octahedral geometry with five water molecules and one phenyl dithiocarbamate ligand coordinated via one of the sulphur atoms (Figure 10). The Na―O bond lengths vary from 2.3562(11) to 2.4247(13) Å and the Na―S bond is 3.0235(6) Å. Most bond lengths and angles are in good agreement with values for comparable compounds whose metrical parameters have been deposited with the Cambridge Structural Database (Table 5) [16]. The exception being the S2=C7 double bond of length 1.7102(13) Å which is longer than the average bond length of 1.65 Å.

Two pairs of water molecules (O1 and O3; O2 and O2 [1-x, 1-y, 1-z]) form bridges with neighbouring sodium atoms forming a 1 D infinite polymeric chain (Figure 1) parallel to the c-axis. The Na to Na distances are 3.4378(8) and 3.5878(8) Å. These parallel polymeric chains form alternating sodium/water and phenyl dithiocarbamate layers parallel to the bc plane as can be seen in the packing diagram Figure 11.

Figure 10. Crystal structure of ORTEP diagram of the sodium phenyl dithiocarbamate structure with ellipsoids drawn at the 50 % probability level. Symmetry codes: i) 1 – x, y, 3/2 – z; ii) 1 – x, 1 – y, 1 – z; iii) x, 1 – y, ½ + z; iv) x, 1 – y, -½ + z.

Figure 10. Crystal structure of ORTEP diagram of the sodium phenyl dithiocarbamate structure with ellipsoids drawn at the 50 % probability level. Symmetry codes: i) 1 – x, y, 3/2 – z; ii) 1 – x, 1 – y, 1 – z; iii) x, 1 – y, ½ + z; iv) x, 1 – y, -½ + z.

Figure 11. Packing diagram of the sodium phenyl dithiocarbamate structure drawn normal to (0 1 0). Ellipsoids drawn at the 50% probability level.

Figure 11. Packing diagram of the sodium phenyl dithiocarbamate structure drawn normal to (0 1 0). Ellipsoids drawn at the 50% probability level.

Most bond lengths and angles are in good agreement with values for comparable compounds whose metrical parameters have been deposited with the Cambridge Structural Database (Table 5) [16].

Within the polymeric chains there are four O―H…S interactions with S2 being trifurcated accepting (Table 5). S1 is also trifurcated accepting with one intramolecular interaction within the polymeric chain and two intermolecular interactions to a neighbouring chain. The S…H interaction lengths vary from 2.39(2) to 2.59(2) Å. There are also N―H…O interactions of 2.24(2) Å between chains. Pairs of phenyl π…π ring interactions of lengths 4.1918(8) and 4.1917(8) Å with dihedral angles of 4.16(7) ° and slippages of 2.436 and 2.661 Å respectively also occur between parallel chains.

Table 5. Selected bond lengths and angles. Symmetry codes: i) 1 – x, y, 3/2 – z; ii) 1 – x, 1 – y, 1 – z.

Table 5. Selected bond lengths and angles. Symmetry codes: i) 1 – x, y, 3/2 – z; ii) 1 – x, 1 – y, 1 – z.

Lengths (Å)		Angles (°)
S1―Na1	3.0235(6)	Na1―S1―C7	109.73(5)
S1―C7	1.7353(15)	Na1―O1―Na1i	93.70(5)
S2―C7	1.7102(13)	Na1―O2―Na1ii	96.37(4)
Na1―O1	2.3562(11)	Na1―O3―Na1i	92.83(5)
Na1―O2	2.3891(12)	S1―Na1―O1	94.73(3)
Na1―O3	2.3730(11)	S1―Na1―O2	84.96(3)
Na1―O4	2.3757(13)	S1―Na1―O3	172.90(3)
Na1―O2ii	2.4247(13)	S1―Na1―O4	81.85(3)
N1―C1	1.4397(19)	S1―Na1―O2ii	86.21(3)
N1―C7	1.3305(18)	O1―Na1―O2	167.50(4)
		O1―Na1―O3	86.74(4)
		O1―Na1―O4	91.95(3)
		O1―Na1―O2ii	83.88(3)
		O2―Na1―O3	92.11(4)
		O2―Na1―O4	100.36(4)
		O2―Na1―O2ii	83.63(4)
		O3―Na1―O4	105.07(3)
		O2ii―Na1―O3	87.04(3)
		O2ii―Na1―O4	167.00(4)
		C1―N1―C7	124.97(12)

Table 6. Hydrogen interactions. * indicates intramolecular interactions within the polymeric chain. Symmetry codes: i) x, -y, -½ + z; ii) 1-x, -y, 1 – z; iii) x, 1 + y, z; iv) x, 1 - y, ½ + z.

Table 6. Hydrogen interactions. * indicates intramolecular interactions within the polymeric chain. Symmetry codes: i) x, -y, -½ + z; ii) 1-x, -y, 1 – z; iii) x, 1 + y, z; iv) x, 1 - y, ½ + z.

D―H…A	D―H (Å)	H…A (Å)	D…A (Å)	D―H…A (°)
N1―H1…O4i	0.84(2)	2.24(2)	3.0205(16)	156.7(17)
O1―H1A…S1ii	0.83(2)	2.43(2)	3.2446(9)	169.3(18)
*O2―H2A…S2	0.84(2)	2.39(2)	3.2162(12)	172.0(18)
O2―H2B…S1iii	0.77(2)	2.59(2)	3.3478(11)	167.2(18)
*O3―H3A…S1iv	0.80(2)	2.52(2)	3.3152(9)	177.5(17)
*O4―H4A…S2	0.80(2)	2.54(2)	3.3145(12)	163(2)
*O4―H4B…S2iv	0.80(2)	2.52(2)	3.2789(12)	158(2)

3.6. Antibacterial Studies and Mechanism of Action

The Kirby-Bauer method also referred to disc diffusion method or Kirby-Bauer disc diffusion method was used to assess how effective the antibacterial actvities of studied compounds were, against four bacterial strains [17,25]. The Mueller-Hinton agar was used to give a good batch-to-batch reproducibility [25]. The bacterial strains are first spread on the agar plate, followed by prepared paper disks of studied compounds. Plates were assessed for their zones of inhibition (ZOI) after incubation at 37^oC at 24 h as shown in Table 7.

In general, the metallic compounds of vanadium(IV) sulfate. hydrate showed no activity against all the four bacterial strains (two Gram-negative bacterial strains of S.aureus and E. coli and two Gram-positive bacterial strains of E. faecalis and P. aeruginosa), while zinc(II) chloride showed towards the two Gram-negative bacterial strains of S.aureus and E. coli with ZOI of 14 mm and 9 mm respectively.

Both ligands (sfz and pl-dtc) were active against S. aureus with zone of inhibition of 17 mm and 8 mm respectively, but the corresponding coordination compounds of [VO(sfz)(pl-dtc)]and [Zn(sfz)(pl-dtc)] were inactive. None of the ligands and coordination compounds was active against E. faecalis. Ligand of sfz was active against E. coli with zone of inhibition of 16.3 mm and pl-dtc with no ZOI, but the corresponding coordination compounds were less active, where [VO(sfz)(pl-dtc)] has 8 mm and [Zn(sfz)(pl-dtc)] has 10 mm. Ligands of sfz and pl-dtc, as well as, coordination compound of [VO(sfz)(pl-dtc)] were not active against P. aeruginosa, but the biological activity of [Zn(sfz)(pl-dtc)] was enhanced when compared with individual ligands of sfz and pl-dtc as shown in Table 7. The enhanced activities of the coordination compounds compared with pl-dtc ligand could be explained on the basis of chelation theory [25]. Chelation theory states that a decrease in the polarizability of the metal could improve the lipophilicity of the coordination compounds [25]. This will result to a breakdown of the permeability of the cells, resulting in interruption with normal cell processes [25].

In summary, both ligands and coordination compounds have potentials as antibacterial agents

Table 7. The zone of inhibition for studied compounds in mm.

Table 7. The zone of inhibition for studied compounds in mm.

Compound	S.aureus MRSA252	E.faecalis ATCC 19433	E. coli MC4100	P.aeruginosa PAO1
VOSO4. H20	0	0	0	0
ZnCl2	14	0	9	0
sfz	17	ND	16.3	ND
pl-dtc	8	ND	0	ND
[VO(sfz)(pl-dtc)]	0	NA	8	NA
[Zn(sfz)(pl-dtc)]	0	NA	10	10
Meropenem	NA	NA	NA	30
Tetracycline	30	NA	28	NA
Vancomycin	NA	22	NA	NA
DMSO	0	0	0	0

sfz = Sodium sulfadiazine; pl-dtc = Sodium phenyl dithiocarbamate; [VO(sfz)(pl-dtc)]= Coordination compound of oxovanadium(IV) ion; [Zn(sfz)(pl-dtc)] = Coordination compound of zinc(II) ion; ND=Non Detectable; NA= Not Active; Negative control: DMSO; Positive controls: Meropenem, Tetracycline and Vancomycin.

4. Conclusion and Future Perspective

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