Version 1
: Received: 13 August 2024 / Approved: 14 August 2024 / Online: 14 August 2024 (14:46:44 CEST)
How to cite:
Belozertseva, I. V.; Merkulovs, D. D.; Kaiser, H.; Rozhdestvensky, T. S.; Skryabin, B. V. Advancing 3Rs: The Mouse Estrus Detector (MED) as a Low-Stress, Painless, and Efficient Tool for Estrus Determination in Mice. Preprints2024, 2024081091. https://doi.org/10.20944/preprints202408.1091.v1
Belozertseva, I. V.; Merkulovs, D. D.; Kaiser, H.; Rozhdestvensky, T. S.; Skryabin, B. V. Advancing 3Rs: The Mouse Estrus Detector (MED) as a Low-Stress, Painless, and Efficient Tool for Estrus Determination in Mice. Preprints 2024, 2024081091. https://doi.org/10.20944/preprints202408.1091.v1
Belozertseva, I. V.; Merkulovs, D. D.; Kaiser, H.; Rozhdestvensky, T. S.; Skryabin, B. V. Advancing 3Rs: The Mouse Estrus Detector (MED) as a Low-Stress, Painless, and Efficient Tool for Estrus Determination in Mice. Preprints2024, 2024081091. https://doi.org/10.20944/preprints202408.1091.v1
APA Style
Belozertseva, I. V., Merkulovs, D. D., Kaiser, H., Rozhdestvensky, T. S., & Skryabin, B. V. (2024). Advancing 3Rs: The Mouse Estrus Detector (MED) as a Low-Stress, Painless, and Efficient Tool for Estrus Determination in Mice. Preprints. https://doi.org/10.20944/preprints202408.1091.v1
Chicago/Turabian Style
Belozertseva, I. V., Timofey S. Rozhdestvensky and Boris V. Skryabin. 2024 "Advancing 3Rs: The Mouse Estrus Detector (MED) as a Low-Stress, Painless, and Efficient Tool for Estrus Determination in Mice" Preprints. https://doi.org/10.20944/preprints202408.1091.v1
Abstract
Determining the estrous cycle stages in mice is essential for optimizing breeding strategies, synchronizing experimental time lines, and facilitating studies in behavior, drug testing, and genetics. It is critical for reducing the production of genetically unmodified offspring in the generation and investigation of genetically modified animal models. An accurate detection of the estrus cycle is particularly relevant in the context of the 3Rs—Replacement, Reduction and Refinement. The estrous cycle, encompassing the reproductive phases of mice, is key to refining experimental designs and addressing ethical issues related to the use of animals in research. This study presents results from two independent laboratories on the efficacy of the Mouse Estrus Detector (MED) from Elmi Ltd. (Latvia) for the accurate determination of the estrus phase. The female mice of five strains/stocks (CD1, FVB/N, C57Bl6/J, B6D2F1, and Swiss) were used. The results showed that the MEDProTM is a low -traumatic, simple, rapid and painless method of estrus detection that supports the principles of the 3Rs. The use of the MEDProTM for estrus detection in mice caused minimal stress, enhanced mating efficiency, facilitated an increase in the number of embryos for the in vitro fertilization and allowed the production of the desired number of foster animals.
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
