Version 1
: Received: 15 August 2024 / Approved: 16 August 2024 / Online: 19 August 2024 (04:30:15 CEST)
How to cite:
Wante, S. P.; Leung, D. W.; Alizadeh, H. Varying Tolerance to Diesel Toxicity Revealed by Growth Response Evaluation of Petunia grandiflora Shoot Lines Regenerated after Diesel Fuel Treatment. Preprints2024, 2024081248. https://doi.org/10.20944/preprints202408.1248.v1
Wante, S. P.; Leung, D. W.; Alizadeh, H. Varying Tolerance to Diesel Toxicity Revealed by Growth Response Evaluation of Petunia grandiflora Shoot Lines Regenerated after Diesel Fuel Treatment. Preprints 2024, 2024081248. https://doi.org/10.20944/preprints202408.1248.v1
Wante, S. P.; Leung, D. W.; Alizadeh, H. Varying Tolerance to Diesel Toxicity Revealed by Growth Response Evaluation of Petunia grandiflora Shoot Lines Regenerated after Diesel Fuel Treatment. Preprints2024, 2024081248. https://doi.org/10.20944/preprints202408.1248.v1
APA Style
Wante, S. P., Leung, D. W., & Alizadeh, H. (2024). Varying Tolerance to Diesel Toxicity Revealed by Growth Response Evaluation of Petunia grandiflora Shoot Lines Regenerated after Diesel Fuel Treatment. Preprints. https://doi.org/10.20944/preprints202408.1248.v1
Chicago/Turabian Style
Wante, S. P., David W.M. Leung and Hossein Alizadeh. 2024 "Varying Tolerance to Diesel Toxicity Revealed by Growth Response Evaluation of Petunia grandiflora Shoot Lines Regenerated after Diesel Fuel Treatment" Preprints. https://doi.org/10.20944/preprints202408.1248.v1
Abstract
Continuous efforts are required to find ways to protect crop production against the toxicity of petroleum hydrocarbons, such as diesel, contamination of soils. There is a need for identification of candidate plants that are tolerant to diesel toxicity that might also have the potential for remediation of diesel-contaminated soils. In this study, petunia, a popular ornamental plant and a model experimental plant in research on phytoremediation of environmental pollutants, was used to evaluate a novel method for rapidly assessing diesel toxicity based on the tolerance of shoots generated through in vitro plamt cell culture selection. Petunia shoot lines (L1 to L4) regenerated from diesel-treated callus were compared with those from non-diesel-treated callus (control). Significant morphological differences were observed among the tested lines and control, notably with L1 and L4 showing superior growth. In particular, L4 exhibited remarkable adaptability, with increased root development and microbial counts in a diesel-contaminated potting mix, suggesting that the shoots exhibited enhanced tolerance to diesel exposure. Here, this rapid bioassay has been shown to effectively identify plants with varying levels of tolerance to diesel toxicity and could therefore assist accelerated selection of superior plants for phytoremediation. Further research is needed to understand the genetic and physiological mechanisms underlying tolerance traits, with potential applications beyond petunias to other environmentally significant plants.
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
