Version 1
: Received: 16 August 2024 / Approved: 17 August 2024 / Online: 20 August 2024 (04:55:15 CEST)
How to cite:
Shin, H.; Lee, S.; Choi, M.-M.; Yi, H.; Chung, Y.-S. Development of Smallpox Antibody Testing and Surveillance Following Smallpox Vaccination in the Republic of Korea. Preprints2024, 2024081291. https://doi.org/10.20944/preprints202408.1291.v1
Shin, H.; Lee, S.; Choi, M.-M.; Yi, H.; Chung, Y.-S. Development of Smallpox Antibody Testing and Surveillance Following Smallpox Vaccination in the Republic of Korea. Preprints 2024, 2024081291. https://doi.org/10.20944/preprints202408.1291.v1
Shin, H.; Lee, S.; Choi, M.-M.; Yi, H.; Chung, Y.-S. Development of Smallpox Antibody Testing and Surveillance Following Smallpox Vaccination in the Republic of Korea. Preprints2024, 2024081291. https://doi.org/10.20944/preprints202408.1291.v1
APA Style
Shin, H., Lee, S., Choi, M. M., Yi, H., & Chung, Y. S. (2024). Development of Smallpox Antibody Testing and Surveillance Following Smallpox Vaccination in the Republic of Korea. Preprints. https://doi.org/10.20944/preprints202408.1291.v1
Chicago/Turabian Style
Shin, H., Hwajung Yi and Yoon-Seok Chung. 2024 "Development of Smallpox Antibody Testing and Surveillance Following Smallpox Vaccination in the Republic of Korea" Preprints. https://doi.org/10.20944/preprints202408.1291.v1
Abstract
Background: Despite its global eradication in 1977, smallpox remains a concern owing to its potential as a biological agent, thereby prompting the ongoing development and utilization of its vaccine. Vaccination with the Vaccinia virus induces immunity against variola virus, the causative agent of smallpox; however, this immunity does not extend to viruses of different genera within the Poxviridae family. In this study, we aimed to assess the efficacy of an enzyme-linked immunosorbent assay (ELISA) method utilizing Vaccinia virus and recombinant A27L antigen for detecting antibodies against smallpox. Methods. Analysis of serum from 20 individuals pre- and post-vaccination with the CJ strain (CJ50300) revealed neutralizing antibodies, which were confirmed using the plaque reduction neutralization test (PRNT). The ELISA method, validated with a PRNT50 cut-off value of >4, exhibited a sensitivity and specificity of >95% and was particularly reactive with the inactivated virus. Furthermore, adherence to the smallpox vaccination policy revealed significant differences in Orthopoxvirus antibody levels among 300 individuals of different age groups. These findings highlight the reliability and efficacy of the ELISA method in detecting post-vaccination antibodies and contribute significantly to diagnostic methods to prepare for potential smallpox resurgence and bioterrorism threats.
Public Health and Healthcare, Public Health and Health Services
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
