Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Production of Immunoglobulin Y Antibodies and Their Application in an Indirect Enzyme-Linked Immunosorbent Assay for the Evaluation of Humoral Immunity in Colossoma macropomum against Flavobacterium oreochromis

Maria Vitória Lobo Corado
,
Elcimara Cardoso Pereira
,
Taísa Freitas Botinelly
,
Juliane Corrêa Glória
,
Rafael Luckwu de Sousa
,
André de Lima Guerra Corado
,
Antônio Alcirley da Silva Balieiro
ORCID logo ,
Luís André Morais Mariúba
,
Silvia Umeda Gallani
,
Guilherme Campos Tavares
* ORCID logo
Version 1 : Received: 20 August 2024 / Approved: 20 August 2024 / Online: 20 August 2024 (11:33:57 CEST)

How to cite: Corado, M. V. L.; Pereira, E. C.; Botinelly, T. F.; Glória, J. C.; de Sousa, R. L.; Corado, A. D. L. G.; Balieiro, A. A. D. S.; Mariúba, L. A. M.; Gallani, S. U.; Tavares, G. C. Production of Immunoglobulin Y Antibodies and Their Application in an Indirect Enzyme-Linked Immunosorbent Assay for the Evaluation of Humoral Immunity in Colossoma macropomum against Flavobacterium oreochromis. Preprints 2024, 2024081454. https://doi.org/10.20944/preprints202408.1454.v1 Corado, M. V. L.; Pereira, E. C.; Botinelly, T. F.; Glória, J. C.; de Sousa, R. L.; Corado, A. D. L. G.; Balieiro, A. A. D. S.; Mariúba, L. A. M.; Gallani, S. U.; Tavares, G. C. Production of Immunoglobulin Y Antibodies and Their Application in an Indirect Enzyme-Linked Immunosorbent Assay for the Evaluation of Humoral Immunity in Colossoma macropomum against Flavobacterium oreochromis. Preprints 2024, 2024081454. https://doi.org/10.20944/preprints202408.1454.v1

Abstract

Infections with Flavobacterium oreochromis pose risks to Colossoma macropomum production. Since no treatment exists, prophylaxis through vaccination is the only form for disease control. The enzyme-linked immunosorbent assay (ELISA) is widely used in aquaculture to assess animal immunity and mainly to validate vaccination protocols. The method based on the use of immunoglobulin Y (IgY) is an alternative to consider due to its advantages over antibodies produced by small mammals. This study aimed to standardize an indirect ELISA (iELISA) protocol to validate the immunization of C. macropomum against F. oreochromis. A hen and fish were immunized to obtain anti-C. macropomum and anti-F. oreochromis antibodies, which were used to standardize the test. The iELISA was composed of the inactivated bacteria in the coating step, C. macropomum anti-Flavobacterium oreochromis as the primary antibody, and anti-C. macropomum IgY as the secondary antibody. The results demonstrate that the highest antibody titers were among the immunized groups, which were bacterin + adjuvant and bacterin. The iELISA enabled the detection of antibodies produced by C. macropomum, which were mediated by IgY specificity.

Keywords

IgY; immunoassay; plasma; tambaqui; vaccine

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.