Version 1
: Received: 20 August 2024 / Approved: 20 August 2024 / Online: 21 August 2024 (08:37:50 CEST)
How to cite:
Vazquez Avendaño, J. R.; Cortez Romero, C.; Ambríz García, D. A.; Rodriguéz Suastegui, J. L.; Hernández Pichardo, J. E.; Navarro Maldonado, M. D. C. Influence of Oviductal and Uterine Fluid Supplementation on the In Vitro Development and Quality of Sheep Cloned Embryos. Preprints2024, 2024081507. https://doi.org/10.20944/preprints202408.1507.v1
Vazquez Avendaño, J. R.; Cortez Romero, C.; Ambríz García, D. A.; Rodriguéz Suastegui, J. L.; Hernández Pichardo, J. E.; Navarro Maldonado, M. D. C. Influence of Oviductal and Uterine Fluid Supplementation on the In Vitro Development and Quality of Sheep Cloned Embryos. Preprints 2024, 2024081507. https://doi.org/10.20944/preprints202408.1507.v1
Vazquez Avendaño, J. R.; Cortez Romero, C.; Ambríz García, D. A.; Rodriguéz Suastegui, J. L.; Hernández Pichardo, J. E.; Navarro Maldonado, M. D. C. Influence of Oviductal and Uterine Fluid Supplementation on the In Vitro Development and Quality of Sheep Cloned Embryos. Preprints2024, 2024081507. https://doi.org/10.20944/preprints202408.1507.v1
APA Style
Vazquez Avendaño, J. R., Cortez Romero, C., Ambríz García, D. A., Rodriguéz Suastegui, J. L., Hernández Pichardo, J. E., & Navarro Maldonado, M. D. C. (2024). Influence of Oviductal and Uterine Fluid Supplementation on the In Vitro Development and Quality of Sheep Cloned Embryos. Preprints. https://doi.org/10.20944/preprints202408.1507.v1
Chicago/Turabian Style
Vazquez Avendaño, J. R., José Ernesto Hernández Pichardo and María del Carmen Navarro Maldonado. 2024 "Influence of Oviductal and Uterine Fluid Supplementation on the In Vitro Development and Quality of Sheep Cloned Embryos" Preprints. https://doi.org/10.20944/preprints202408.1507.v1
Abstract
Somatic cell nuclear transfer (SCNT) has great potential in the replication of high commercial value animals, threatened wild species for conservation purposes, and transgenic animals for biomedical purposes. However, SCNT has a low success rate, due to intrinsic factors of the technique itself, which will lead to low rates of embryonic development and epigenetic alterations in the cloned embryos. The objective of the present study was to evaluate the effect of OF-UF on the intracellular concentration of ROS, GSH, and the development of Ovis aries cloned and parthenogenetic embryos. The results did not show a beneficial effect on the development of parthenogenetic and cloned embryos at concentrations of 0.5% OF-UF. Furthermore, at 1% OF-UF, an adverse effect was observed in cloned embryos at the blastocyst stage and 2% OF and UF in parthenogenetic embryos in the first divisions. A decrease in ROS and GSH levels was observed in the parthenogenetic blastocysts treated with 1% OF-UF, but not in the clones, in which a higher concentration of GSH and a similar concentration of ROS was observed. No effect was observed of OF-UF on embryonic development and redox balance on sheep clone embryos by Handmade Cloning.
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
