Version 1
: Received: 20 August 2024 / Approved: 21 August 2024 / Online: 21 August 2024 (11:33:03 CEST)
How to cite:
Chung, N.; Lee, D. J.; Lee, S. J.; Kang, G.; Park, M.; Joo, Y. H. Performance Evaluation of Biowashing Pilot Reactor for Remediation of Crude Oil-contaminated Soil of Kuwait. Preprints2024, 2024081529. https://doi.org/10.20944/preprints202408.1529.v1
Chung, N.; Lee, D. J.; Lee, S. J.; Kang, G.; Park, M.; Joo, Y. H. Performance Evaluation of Biowashing Pilot Reactor for Remediation of Crude Oil-contaminated Soil of Kuwait. Preprints 2024, 2024081529. https://doi.org/10.20944/preprints202408.1529.v1
Chung, N.; Lee, D. J.; Lee, S. J.; Kang, G.; Park, M.; Joo, Y. H. Performance Evaluation of Biowashing Pilot Reactor for Remediation of Crude Oil-contaminated Soil of Kuwait. Preprints2024, 2024081529. https://doi.org/10.20944/preprints202408.1529.v1
APA Style
Chung, N., Lee, D. J., Lee, S. J., Kang, G., Park, M., & Joo, Y. H. (2024). Performance Evaluation of Biowashing Pilot Reactor for Remediation of Crude Oil-contaminated Soil of Kuwait. Preprints. https://doi.org/10.20944/preprints202408.1529.v1
Chicago/Turabian Style
Chung, N., Minkoo Park and Young Hoon Joo. 2024 "Performance Evaluation of Biowashing Pilot Reactor for Remediation of Crude Oil-contaminated Soil of Kuwait" Preprints. https://doi.org/10.20944/preprints202408.1529.v1
Abstract
Bioremediation of crude oil-contaminated soil in Kuwait was evaluated using the biowashing pilot reactor system, whose components included 3 biowashing reactors; an oil separator, a hydrocyclone, and a dissolved air flotation. The biowashing pilot reactor system was fed with hemoglobin, a cheap and rich nutrient source containing carbon and nitrogen for bacterial growth. The initial total petroleum hydrocarbons (TPH) concentration was about 84,000 mg/kg soil. The initial TPH concentration decreased to 38,000 mg/kg soil on day 1. The degradation extents of TPH were 55%, 91%, and 96% on days 1, 3, and 5. The first-order rate constant for TPH degradation rate was 0.6820.0004/day. The initial unresolved complex mixture (UCM) concentration was 78,000 mg/kg soil. The degradation extents of UCM were 53%, 91%, and 98% on days 1, 3, and 5. Then, the degradation extents of individual components of total polycyclic aromatic hydrocarbons (PAH), alkylated PAH, and n-alkanes were measured for 5 days. 16S rRNA gene copy number was measured during 5 days for bacterial population estimation. Although there was a day delay in the accretion of the number of copies, the number increased from day 2 to day 5. The present study suggests that the biowashing pilot reactor system with a capacity of 200 L is efficient for TPH degradation.
Keywords
bioremediation, biowashing, crude oil, pilot reactor, total petroleum hydrocarbon
Subject
Environmental and Earth Sciences, Pollution
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
