PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Assessment of Wound Bed Delivery of Galectin-3 to Modulate Macrophage Polarization, Re-epithelialization and Collagen Synthesis in a Murine Model of Excisional Skin Healing
Version 1
: Received: 21 August 2024 / Approved: 22 August 2024 / Online: 22 August 2024 (11:43:18 CEST)
How to cite:
McLeod, K. M.; Dr Gregerio, M.; Tinney, D.; Carmichael, J. J.; Zuanazzi, D.; Siqueira, W. L.; Rizkalla, A.; Hamilton, D. W. Assessment of Wound Bed Delivery of Galectin-3 to Modulate Macrophage Polarization, Re-epithelialization and Collagen Synthesis in a Murine Model of Excisional Skin Healing. Preprints2024, 2024081653. https://doi.org/10.20944/preprints202408.1653.v1
McLeod, K. M.; Dr Gregerio, M.; Tinney, D.; Carmichael, J. J.; Zuanazzi, D.; Siqueira, W. L.; Rizkalla, A.; Hamilton, D. W. Assessment of Wound Bed Delivery of Galectin-3 to Modulate Macrophage Polarization, Re-epithelialization and Collagen Synthesis in a Murine Model of Excisional Skin Healing. Preprints 2024, 2024081653. https://doi.org/10.20944/preprints202408.1653.v1
McLeod, K. M.; Dr Gregerio, M.; Tinney, D.; Carmichael, J. J.; Zuanazzi, D.; Siqueira, W. L.; Rizkalla, A.; Hamilton, D. W. Assessment of Wound Bed Delivery of Galectin-3 to Modulate Macrophage Polarization, Re-epithelialization and Collagen Synthesis in a Murine Model of Excisional Skin Healing. Preprints2024, 2024081653. https://doi.org/10.20944/preprints202408.1653.v1
APA Style
McLeod, K. M., Dr Gregerio, M., Tinney, D., Carmichael, J. J., Zuanazzi, D., Siqueira, W. L., Rizkalla, A., & Hamilton, D. W. (2024). Assessment of Wound Bed Delivery of Galectin-3 to Modulate Macrophage Polarization, Re-epithelialization and Collagen Synthesis in a Murine Model of Excisional Skin Healing. Preprints. https://doi.org/10.20944/preprints202408.1653.v1
Chicago/Turabian Style
McLeod, K. M., Amin Rizkalla and Douglas William Hamilton. 2024 "Assessment of Wound Bed Delivery of Galectin-3 to Modulate Macrophage Polarization, Re-epithelialization and Collagen Synthesis in a Murine Model of Excisional Skin Healing" Preprints. https://doi.org/10.20944/preprints202408.1653.v1
Abstract
Chronic wounds remain trapped in a pro-inflammatory state, with strategies targeted at inducing re-epithelialization and the proliferative phase of healing desirable. A member of the lectin family, galec-tin-3 is implicated in regulation of macrophage phenotype and epithelial migration. We investigated if local delivery of galectin-3 enhanced skin healing in a full thickness excisional C57BL/6 mouse model. An elec-trospun gelatin scaffold loaded with galectin-3 was developed and compared to topical delivery of galec-tin-3. Electrospun gelatin/galectin-3 scaffolds had an average fiber diameter of 200 nm, with 83% scaffold porosity approximately and am average pore diameter of 1.15 μm. The developed scaffolds supported der-mal fibroblasts adhesion, matrix deposition and proliferation in vitro. In vivo treatment of 6 mm full thickness excisional wounds with gelatin/galectin-3 scaffolds did not influence wound closure, re-epithelialization or macrophage phenotypes, but increased collagen synthesis. In comparison, topical delivery of galectin-3 [6.7 µg/ml] significantly increased arginase-I cell density at day 7 versus untreated and gelatin/galectin-3 scaf-folds (p
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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