Version 1
: Received: 22 August 2024 / Approved: 22 August 2024 / Online: 23 August 2024 (11:19:22 CEST)
How to cite:
Yamada, S.; Warashina, T.; Shirota, O.; Kato, Y.; Fukuda, T. Identification of Petit Vert Leaf-Derived Sinapic Acid Derivatives and Their Enhancement of Glucose Uptake in C2C12 Murine Myoblasts. Preprints2024, 2024081666. https://doi.org/10.20944/preprints202408.1666.v1
Yamada, S.; Warashina, T.; Shirota, O.; Kato, Y.; Fukuda, T. Identification of Petit Vert Leaf-Derived Sinapic Acid Derivatives and Their Enhancement of Glucose Uptake in C2C12 Murine Myoblasts. Preprints 2024, 2024081666. https://doi.org/10.20944/preprints202408.1666.v1
Yamada, S.; Warashina, T.; Shirota, O.; Kato, Y.; Fukuda, T. Identification of Petit Vert Leaf-Derived Sinapic Acid Derivatives and Their Enhancement of Glucose Uptake in C2C12 Murine Myoblasts. Preprints2024, 2024081666. https://doi.org/10.20944/preprints202408.1666.v1
APA Style
Yamada, S., Warashina, T., Shirota, O., Kato, Y., & Fukuda, T. (2024). Identification of Petit Vert Leaf-Derived Sinapic Acid Derivatives and Their Enhancement of Glucose Uptake in C2C12 Murine Myoblasts. Preprints. https://doi.org/10.20944/preprints202408.1666.v1
Chicago/Turabian Style
Yamada, S., Yoshihisa Kato and Toshiyuki Fukuda. 2024 "Identification of Petit Vert Leaf-Derived Sinapic Acid Derivatives and Their Enhancement of Glucose Uptake in C2C12 Murine Myoblasts" Preprints. https://doi.org/10.20944/preprints202408.1666.v1
Abstract
Petit vert (scientific name: Brassica oleracea var. gemmifera DC. × Brassica oleracea var. acephala DC.) is a new variety of vegetable created by crossbreeding kale and brussels sprouts (Brassica oleracea species). The present study aimed to identify biologically active compounds in extracts of the outer leaves of Petit vert by purification and to examine their biological activities. Dried and powdered outer leaves of Petit vert were extracted, fractionated, and purified to isolate active compounds. Nuclear magnetic resonance and mass spectrometries were performed to elucidate the structures of five compounds (1‒5). The compounds isolated from Petit vert leaves were glycosides that contained kaempferol, quercetin (flavonol), or sinapinic acid (phenylpropanoid). Glucose uptake in cultured C2C12 murine myoblasts in the absence of insulin was significantly increased by these compounds, kaempferol, sinapic acid, and ferulic acid, while that in the presence of insulin was also significantly increased by compounds 3 and 4, kaempferol, and sinapic acid. The present study reports for the first time the isolation of five compounds containing sinapic acid from the outer leaves of Petit vert and their stimulation of glucose uptake in cultured C2C12 murine myoblasts. The results obtained herein suggest the potential of these compounds to effectively attenuate hyperglycemia and maintain muscle strength by promoting glucose metabolism in muscle cells.
Keywords
Petit vert leaves; sinapic acid; glucose uptake
Subject
Medicine and Pharmacology, Complementary and Alternative Medicine
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
