Version 1
: Received: 22 August 2024 / Approved: 23 August 2024 / Online: 23 August 2024 (08:23:30 CEST)
How to cite:
Mania, S.; Banach-Kopeć, A.; Maciejewska, N.; Czerwiec, K.; Słonimska, P.; Deptuła, M.; Baczyński-Keller, J.; Pikuła, M.; Sachadyn, P.; Tylingo, R. From Bioink to Tissue: Exploring Chitosan-Agarose Composite in the Context of Printability and Cellular Behaviour. Preprints2024, 2024081701. https://doi.org/10.20944/preprints202408.1701.v1
Mania, S.; Banach-Kopeć, A.; Maciejewska, N.; Czerwiec, K.; Słonimska, P.; Deptuła, M.; Baczyński-Keller, J.; Pikuła, M.; Sachadyn, P.; Tylingo, R. From Bioink to Tissue: Exploring Chitosan-Agarose Composite in the Context of Printability and Cellular Behaviour. Preprints 2024, 2024081701. https://doi.org/10.20944/preprints202408.1701.v1
Mania, S.; Banach-Kopeć, A.; Maciejewska, N.; Czerwiec, K.; Słonimska, P.; Deptuła, M.; Baczyński-Keller, J.; Pikuła, M.; Sachadyn, P.; Tylingo, R. From Bioink to Tissue: Exploring Chitosan-Agarose Composite in the Context of Printability and Cellular Behaviour. Preprints2024, 2024081701. https://doi.org/10.20944/preprints202408.1701.v1
APA Style
Mania, S., Banach-Kopeć, A., Maciejewska, N., Czerwiec, K., Słonimska, P., Deptuła, M., Baczyński-Keller, J., Pikuła, M., Sachadyn, P., & Tylingo, R. (2024). From Bioink to Tissue: Exploring Chitosan-Agarose Composite in the Context of Printability and Cellular Behaviour. Preprints. https://doi.org/10.20944/preprints202408.1701.v1
Chicago/Turabian Style
Mania, S., Paweł Sachadyn and Robert Tylingo. 2024 "From Bioink to Tissue: Exploring Chitosan-Agarose Composite in the Context of Printability and Cellular Behaviour" Preprints. https://doi.org/10.20944/preprints202408.1701.v1
Abstract
This study presents an innovative method for producing thermosensitive bio-ink from chitosan hydrogels saturated with carbon dioxide and agarose. It focuses on a detailed characterization of their physicochemical properties and potential applications in biomedicine and tissue engineering. The ORO test approved rapid regeneration of the three-dimensional structure of chitosan-agarose composites in the unidirectional bench press simulation test. The diffusion of dyes through chitosan-agarose hydrogel membranes strongly depended on the share of both polymers in the composite and the molecular weight of the dyes. Glucose, as a nutrient marker, also diffused through all membranes regardless of composition. Biocompatibility assessment using MTT tests on 46BR.1N fibroblasts and HaCaT keratinocytes confirmed the safety of the bioink. The regenerative potential of the bioink was confirmed by efficient cell migration, especially HaCaT. Long-term viability studies showed that chitosan-agarose scaffolds, unlike the agarose ones, support cell proliferation and survival, especially 14 days after bioink extrusion. Experiments in a skin wound model in mice confirmed the biocompatibility of tested dressing and the beneficial action of chitosan on healing. Studies on vessel formation in chicken embryos highlight the potential of the chitosan-agarose composition for enhancing proangiogenic effects. This composition meets all entry criteria and possesses excellent biological properties.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
