Lee, Y.-H.; Jung, J.; Hong, J.-Y. Intratumoral Microbes in Oral Squamous Cell Carcinoma: Focus on Treponema denticola, Lactobacillus casei, and Candida albicans. Preprints2024, 2024081912. https://doi.org/10.20944/preprints202408.1912.v1
APA Style
Lee, Y. H., Jung, J., & Hong, J. Y. (2024). Intratumoral Microbes in Oral Squamous Cell Carcinoma: Focus on <em>Treponema denticola</em>,<em> Lactobacillus casei</em>, and <em>Candida albicans</em>. Preprints. https://doi.org/10.20944/preprints202408.1912.v1
Chicago/Turabian Style
Lee, Y., Junho Jung and Ji-Youn Hong. 2024 "Intratumoral Microbes in Oral Squamous Cell Carcinoma: Focus on <em>Treponema denticola</em>,<em> Lactobacillus casei</em>, and <em>Candida albicans</em>" Preprints. https://doi.org/10.20944/preprints202408.1912.v1
Abstract
Aim:
In this study, we aimed to explore the oral bacteria and fungi that can help discern oral squamous cell carcinoma (OSCC) and investigate the correlations between multiple key pathogens.
Methods:
Twelve participants (8 females and 4 males; mean age, 54.33 ± 20.65 years) were prospectively recruited into three groups: Group 1: healthy control, Group 2: patients with stomatitis, and Group 3: patients with OSCC, with 4 individuals in each group. Unstimulated whole saliva samples from these participants were analyzed using real-time PCR to assess the presence and abundance of 14 major oral bacterial species and Candida albicans.
Results:
The analysis revealed significant differences for certain microorganisms, namely Treponema denticola (T. denticola), Lactobacillus casei (L. casei), and Candida albicans. T. denticola was most abundant in the OSCC group (5,358,692.95 ± 3,540,767.33), compared to the stomatitis (123,355.54 ± 197,490.86) and healthy control (9,999.21 ± 11,998.40) groups. L. casei was undetectable in the healthy control group, but was significantly more abundant in the stomatitis group (1,653.94 ± 2,981.98) and even higher in the OSCC group (21,336.95 ± 9,258.79) (p = 0.001). A similar trend was observed for C. albicans, with DNA copy numbers rising from the healthy control (464.29 ± 716.76) to the stomatitis (1,861.30 ± 1,206.15) to the OSCC group (9,347.98 ± 5,128.54) (p = 0.006). The amount of T. denticola was positively correlated with L. casei (r=0.890, p
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
