Version 1
: Received: 28 August 2024 / Approved: 29 August 2024 / Online: 29 August 2024 (17:14:41 CEST)
How to cite:
Zakaria, M.; Matta, J.; Honjol, Y.; Schupbach, D.; Mwale, F.; Harvey, E.; Merle, G. Decoding Cold Mechanisms of Enhanced Bone Repair through Sensory Receptors and Molecular Pathways. Preprints2024, 2024082177. https://doi.org/10.20944/preprints202408.2177.v1
Zakaria, M.; Matta, J.; Honjol, Y.; Schupbach, D.; Mwale, F.; Harvey, E.; Merle, G. Decoding Cold Mechanisms of Enhanced Bone Repair through Sensory Receptors and Molecular Pathways. Preprints 2024, 2024082177. https://doi.org/10.20944/preprints202408.2177.v1
Zakaria, M.; Matta, J.; Honjol, Y.; Schupbach, D.; Mwale, F.; Harvey, E.; Merle, G. Decoding Cold Mechanisms of Enhanced Bone Repair through Sensory Receptors and Molecular Pathways. Preprints2024, 2024082177. https://doi.org/10.20944/preprints202408.2177.v1
APA Style
Zakaria, M., Matta, J., Honjol, Y., Schupbach, D., Mwale, F., Harvey, E., & Merle, G. (2024). Decoding Cold Mechanisms of Enhanced Bone Repair through Sensory Receptors and Molecular Pathways. Preprints. https://doi.org/10.20944/preprints202408.2177.v1
Chicago/Turabian Style
Zakaria, M., Edward Harvey and Geraldine Merle. 2024 "Decoding Cold Mechanisms of Enhanced Bone Repair through Sensory Receptors and Molecular Pathways" Preprints. https://doi.org/10.20944/preprints202408.2177.v1
Abstract
Applying cold to a bone injury can aid healing, though its mechanisms are complex. This study investigates how cold therapy impacts bone repair to optimize healing. Cold was applied to a rodent bone model, with physiological responses analyzed. Vasoconstriction was mediated by an increase in TRP channels, TRPA1 (p=0.012) and TRPM8 (p<0.001), within cortical defects enhancing sensory response and blood flow regulation. Cold exposure also elevated hypoxia (p < 0.01) and VEGF expression (p < 0.001), promoting angiogenesis vital for bone regeneration. Increased expression of osteogenic proteins PGC-1α (p = 0.039) and RBM3 (p<0.008) suggests stimulated reparative processes. Enhanced osteoblast differentiation and ALP presence at days 5 (three-fold, p=0.014) and 10 (two-fold, p=0.010) were observed, along with increased osteocalcin (OCN) at day 10 (two-fold, p = 0.010) indicating presence of mature osteoblasts capable of mineralization. These findings highlight cold therapy's multifaceted effects on bone repair, offering insights for therapeutic strategies.
Medicine and Pharmacology, Orthopedics and Sports Medicine
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
