Version 1
: Received: 30 August 2024 / Approved: 2 September 2024 / Online: 2 September 2024 (10:57:02 CEST)
How to cite:
Rodríguez-Castillo, A. J.; González-Chávez, S. A.; Portillo-Pantoja, I.; Cruz-Hermosillo, E.; Pacheco-Tena, C.; Chávez-Flores, D.; Delgado-Gardea, M. C. E.; Infante-Ramírez, R.; Sánchez-Ramírez, B. E. Aqueous Extracts of Rhus trilobata Inhibit the LPS-Induced Inflammatory Response In Vitro and In Vivo. Preprints2024, 2024090092. https://doi.org/10.20944/preprints202409.0092.v1
Rodríguez-Castillo, A. J.; González-Chávez, S. A.; Portillo-Pantoja, I.; Cruz-Hermosillo, E.; Pacheco-Tena, C.; Chávez-Flores, D.; Delgado-Gardea, M. C. E.; Infante-Ramírez, R.; Sánchez-Ramírez, B. E. Aqueous Extracts of Rhus trilobata Inhibit the LPS-Induced Inflammatory Response In Vitro and In Vivo. Preprints 2024, 2024090092. https://doi.org/10.20944/preprints202409.0092.v1
Rodríguez-Castillo, A. J.; González-Chávez, S. A.; Portillo-Pantoja, I.; Cruz-Hermosillo, E.; Pacheco-Tena, C.; Chávez-Flores, D.; Delgado-Gardea, M. C. E.; Infante-Ramírez, R.; Sánchez-Ramírez, B. E. Aqueous Extracts of Rhus trilobata Inhibit the LPS-Induced Inflammatory Response In Vitro and In Vivo. Preprints2024, 2024090092. https://doi.org/10.20944/preprints202409.0092.v1
APA Style
Rodríguez-Castillo, A. J., González-Chávez, S. A., Portillo-Pantoja, I., Cruz-Hermosillo, E., Pacheco-Tena, C., Chávez-Flores, D., Delgado-Gardea, M. C. E., Infante-Ramírez, R., & Sánchez-Ramírez, B. E. (2024). Aqueous Extracts of Rhus trilobata Inhibit the LPS-Induced Inflammatory Response In Vitro and In Vivo. Preprints. https://doi.org/10.20944/preprints202409.0092.v1
Chicago/Turabian Style
Rodríguez-Castillo, A. J., Rocío Infante-Ramírez and Blanca Estela Sánchez-Ramírez. 2024 "Aqueous Extracts of Rhus trilobata Inhibit the LPS-Induced Inflammatory Response In Vitro and In Vivo" Preprints. https://doi.org/10.20944/preprints202409.0092.v1
Abstract
Chronic noncommunicable diseases (NCDs) are responsible for approximately 74% of deaths globally. Medicinal plants have traditionally been used to treat NCDs, including diabetes, cancer, and rheumatic diseases, and are a source of anti-inflammatory compounds. This study aimed to evaluate the anti-inflammatory effects of Rhus trilobata (Rt) extracts and fractions in lipopolysaccharide (LPS)-induced inflammation models in vitro and in vivo. The aqueous extract (RtAE) and five fractions (F2 to F6) were obtained via C18 solid-phase separation and tested in murine LPS-induced J774.1 macrophages. Key inflammatory markers such as IL-1β, IL-6, TNF-α, and COX-2 gene expression were measured using RT-qPCR, and PGE2 production was assessed via HPLC-DAD. The in vivo effects were tested in an LPS-induced paw edema model in Wistar rats. Results showed that RtAE at 15 μg/mL significantly decreased IL-1β and IL-6 gene expression in vitro. Fraction F6 further reduced IL-1β, TNF-α, and IL-6 gene expression, COX-2 expression, and PGE2 production. In vivo, F6 significantly reduced LPS-induced paw edema, inflammatory infiltration, and IL-1β and COX-2 protein expression. These findings support the anti-inflammatory potential of RtAE and F6, reinforcing the medicinal use of Rt.
Medicine and Pharmacology, Complementary and Alternative Medicine
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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