Version 1
: Received: 2 September 2024 / Approved: 2 September 2024 / Online: 3 September 2024 (11:50:59 CEST)
How to cite:
Song, Q.; Xu, J.; Shi, H.; Zhang, W.; Cao, H.; Zhai, L. Identification of Candidate Genes for Cold Tolerance at Seedling Stage by GWAS in Rice (Oryza sativa L.). Preprints2024, 2024090227. https://doi.org/10.20944/preprints202409.0227.v1
Song, Q.; Xu, J.; Shi, H.; Zhang, W.; Cao, H.; Zhai, L. Identification of Candidate Genes for Cold Tolerance at Seedling Stage by GWAS in Rice (Oryza sativa L.). Preprints 2024, 2024090227. https://doi.org/10.20944/preprints202409.0227.v1
Song, Q.; Xu, J.; Shi, H.; Zhang, W.; Cao, H.; Zhai, L. Identification of Candidate Genes for Cold Tolerance at Seedling Stage by GWAS in Rice (Oryza sativa L.). Preprints2024, 2024090227. https://doi.org/10.20944/preprints202409.0227.v1
APA Style
Song, Q., Xu, J., Shi, H., Zhang, W., Cao, H., & Zhai, L. (2024). Identification of Candidate Genes for Cold Tolerance at Seedling Stage by GWAS in Rice (Oryza sativa L.). Preprints. https://doi.org/10.20944/preprints202409.0227.v1
Chicago/Turabian Style
Song, Q., Huimin Cao and Laiyuan Zhai. 2024 "Identification of Candidate Genes for Cold Tolerance at Seedling Stage by GWAS in Rice (Oryza sativa L.)" Preprints. https://doi.org/10.20944/preprints202409.0227.v1
Abstract
Due to global climate change, cold temperatures have significantly impacted rice production, resulting in reduced yield and quality. In this study, we investigated two traits related to cold tolerance (CT) of 1992 diverse rice accessions at the seedling stage. Geng accessions exhibited higher levels of CT compared to xian accessions, with the GJ-tmp subgroup displaying the strongest CT. However, extreme CT accessions were also identified within the xian subspecies. Through GWAS analysis based on survival rate (SR) and leaf score of cold tolerance (SCT), a total of 30 QTLs associated with CT at the seedling stage were identified, among which four QTLs (qSR3.1a, qSR4.1a, qSR11.1x and qSR12.1a) were found to be important. Furthermore, five candidate genes (LOC_Os03g44760, LOC_Os04g06900, LOC_Os04g07260, LOC_Os11g40610 and LOC_Os12g10710) along with their favorable haplotypes were identified through gene function annotation and haplotype analysis. Pyramiding multiple favorable haplotypes resulted in a significant improvement in CT performance. Subsequently, three selected accessions (CX534, B236, and IRIS_313-8565), carrying different superior alleles for CT, were selected and recommended for molecular breeding for CT using marker-assisted selection (MAS). The findings from this study provide valuable resources for enhancing rice's ability for CT while laying a foundation for future cloning of novel genes involved in conferring CT.
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
