Version 1
: Received: 3 September 2024 / Approved: 4 September 2024 / Online: 5 September 2024 (05:03:29 CEST)
How to cite:
Munoz-Ballester, C.; Golf, S.; Heithoff, B.; George, K.; Robel, S. Assessment of Blood-Brain Barrier Integrity Using Dyes and Tracers. Preprints2024, 2024090341. https://doi.org/10.20944/preprints202409.0341.v1
Munoz-Ballester, C.; Golf, S.; Heithoff, B.; George, K.; Robel, S. Assessment of Blood-Brain Barrier Integrity Using Dyes and Tracers. Preprints 2024, 2024090341. https://doi.org/10.20944/preprints202409.0341.v1
Munoz-Ballester, C.; Golf, S.; Heithoff, B.; George, K.; Robel, S. Assessment of Blood-Brain Barrier Integrity Using Dyes and Tracers. Preprints2024, 2024090341. https://doi.org/10.20944/preprints202409.0341.v1
APA Style
Munoz-Ballester, C., Golf, S., Heithoff, B., George, K., & Robel, S. (2024). Assessment of Blood-Brain Barrier Integrity Using Dyes and Tracers. Preprints. https://doi.org/10.20944/preprints202409.0341.v1
Chicago/Turabian Style
Munoz-Ballester, C., Kijana George and Stefanie Robel. 2024 "Assessment of Blood-Brain Barrier Integrity Using Dyes and Tracers" Preprints. https://doi.org/10.20944/preprints202409.0341.v1
Abstract
Under physiological conditions the blood-brain barrier (BBB) is required for regulating the passage of substances between the bloodstream and the brain. To date, disruption of the BBB has been associated with nearly all neurological diseases. Dye and tracer experiments have been used to assess BBB integrity for decades, yet quantitative assessment of these experiments has only recently been implemented. Further, interpretation of these results requires the consideration of tracer-specific properties. Here, we describe a protocol for evaluating BBB leakage using the small molecule cadaverine in quantitative way. We also cover advantages/disadvantages of using some reagents and highlight limitations in data interpretations based on assays used to assess BBB permeability.
Biology and Life Sciences, Neuroscience and Neurology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
