Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Depicting of DNA Makeup of Spring Bread Wheat Genotypes Using SSR Markers and Their Association with Heat Tolerance

Version 1 : Received: 6 September 2024 / Approved: 9 September 2024 / Online: 11 September 2024 (10:39:46 CEST)

How to cite: Amin, M. F.; Alam, M. N.; Faruq, G.; Khan, M. M.; Rahman, M. M. Depicting of DNA Makeup of Spring Bread Wheat Genotypes Using SSR Markers and Their Association with Heat Tolerance. Preprints 2024, 2024090824. https://doi.org/10.20944/preprints202409.0824.v1 Amin, M. F.; Alam, M. N.; Faruq, G.; Khan, M. M.; Rahman, M. M. Depicting of DNA Makeup of Spring Bread Wheat Genotypes Using SSR Markers and Their Association with Heat Tolerance. Preprints 2024, 2024090824. https://doi.org/10.20944/preprints202409.0824.v1

Abstract

Wheat is threatened by global warming. This study aimed to characterize based on DNA profile and identify heat-tolerant wheat genotypes. Fifteen wheat genotypes were sown in Nov 21 and Dec 21 and evaluated within 13 SSR markers. A dendrogram classified the genotypes into two main groups. The highest genetic distances (0.93) were drawn between BAW 1290 and BARI Gom 28, and the lowest one between Nadi 2 and BAW 1147. Heat susceptibility index (HSI) values for 1000-grain weight (TGW) and grain yield indicated moderate heat tolerance in Cluster I genotypes (HSI 0.50-1.00), closely matching Group A's Cluster II members. In Group B, Cluster III genotypes had higher HSI (1.04-1.23) and significant reductions in TGW and grain yield under heat stress, indicating heat susceptibility. The varieties BARI Gom 25, 26, 27, 28, 29, and 30 were identified as heat-stress tolerant. This research provides insights for future heat stress-molecular breeding programs.

Keywords

Bread wheat; Fingerprinting; Genetic diversity; Heat tolerance; SSR marker

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

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