Gaurav, S.; Ajit, G.; Esha, R.; Hardik, S.; Kashyap, T.; Sahoo, D.; Patel, A.; Schmidt, J. E. Optimized Polyhydroxybutyrate Production by Neobacillus Niacinii GS1 Utilizing Corn Flour, Wheat Bran, and Peptone: A Sustainable Approach. Preprints2024, 2024091106. https://doi.org/10.20944/preprints202409.1106.v1
APA Style
Gaurav, S., Ajit, G., Esha, R., Hardik, S., Kashyap, T., Sahoo, D., Patel, A., & Schmidt, J. E. (2024). Optimized Polyhydroxybutyrate Production by <em>Neobacillus Niacinii</em> GS1 Utilizing Corn Flour, Wheat Bran, and Peptone: A Sustainable Approach. Preprints. https://doi.org/10.20944/preprints202409.1106.v1
Chicago/Turabian Style
Gaurav, S., Ashish Patel and Jens Ejbye Schmidt. 2024 "Optimized Polyhydroxybutyrate Production by <em>Neobacillus Niacinii</em> GS1 Utilizing Corn Flour, Wheat Bran, and Peptone: A Sustainable Approach" Preprints. https://doi.org/10.20944/preprints202409.1106.v1
Abstract
Plastic pollution is a pressing environmental challenge, necessitating the development of biodegradable alternatives like polyhydroxybutyrate (PHB). This study focuses on optimizing PHB production by Neobacillus niacinii GS1, a bacterium isolated from a municipal dumping site. By utilizing agricultural residues such as corn flour, wheat bran, and peptone as substrates, we aimed to establish an eco-friendly method for biopolymer production, contributing to sustainable waste management and bioplastic innovation. The bacterium was identified using morphological, biochemical, and molecular techniques. The optimization process involved adjusting variables such as inoculum age, inoculum size, incubation time, agitation rate, incubation temperature, pH of the medium, carbon sources and nitrogen sources. Response surface methodology (RSM) was employed to identify optimal conditions, with the highest PHB yield of 61.1% achieved under specific conditions: 37°C, pH 7, and an agitation rate of 150 rpm. These findings underscore the potential of Neobacillus niacinii GS1 in converting agro-industrial residues into valuable biopolymers, promoting sustainable bioplastic production, and advancing waste valorization efforts through the use of eco-friendly materials.
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
