PreprintArticleVersion 1This version is not peer-reviewed
Expressions of Immune Prophenoloxidase (proPO) System-Related Genes to Oxidative Stress in the Gonad and Stomach of the Mud Crab (Macrophthalmus japonicus) Exposed to Endocrine-Disrupting Chemicals
Version 1
: Received: 13 September 2024 / Approved: 14 September 2024 / Online: 14 September 2024 (10:53:35 CEST)
How to cite:
Kim, J.-H.; Park, K.; Kim, W.-S.; Kwak, I.-S. Expressions of Immune Prophenoloxidase (proPO) System-Related Genes to Oxidative Stress in the Gonad and Stomach of the Mud Crab (Macrophthalmus japonicus) Exposed to Endocrine-Disrupting Chemicals. Preprints2024, 2024091132. https://doi.org/10.20944/preprints202409.1132.v1
Kim, J.-H.; Park, K.; Kim, W.-S.; Kwak, I.-S. Expressions of Immune Prophenoloxidase (proPO) System-Related Genes to Oxidative Stress in the Gonad and Stomach of the Mud Crab (Macrophthalmus japonicus) Exposed to Endocrine-Disrupting Chemicals. Preprints 2024, 2024091132. https://doi.org/10.20944/preprints202409.1132.v1
Kim, J.-H.; Park, K.; Kim, W.-S.; Kwak, I.-S. Expressions of Immune Prophenoloxidase (proPO) System-Related Genes to Oxidative Stress in the Gonad and Stomach of the Mud Crab (Macrophthalmus japonicus) Exposed to Endocrine-Disrupting Chemicals. Preprints2024, 2024091132. https://doi.org/10.20944/preprints202409.1132.v1
APA Style
Kim, J. H., Park, K., Kim, W. S., & Kwak, I. S. (2024). Expressions of Immune Prophenoloxidase (proPO) System-Related Genes to Oxidative Stress in the Gonad and Stomach of the Mud Crab (Macrophthalmus japonicus) Exposed to Endocrine-Disrupting Chemicals. Preprints. https://doi.org/10.20944/preprints202409.1132.v1
Chicago/Turabian Style
Kim, J., Won-Seok Kim and Ihn-Sil Kwak. 2024 "Expressions of Immune Prophenoloxidase (proPO) System-Related Genes to Oxidative Stress in the Gonad and Stomach of the Mud Crab (Macrophthalmus japonicus) Exposed to Endocrine-Disrupting Chemicals" Preprints. https://doi.org/10.20944/preprints202409.1132.v1
Abstract
Endocrine-disrupting chemicals (EDCs) significantly damage biological systems related to reproductive, neurological, and metabolic functions. Approximately 1,000 chemicals are known to possess endocrine-acting properties, including bisphenol A (BPA) and di(2-ethylhexyl) phthalate (DEHP). This study primarily focuses on the potential effects of EDCs on the transcriptional levels of innate immune prophenoloxidase (proPO) system-related genes to oxidative stress in the gonad and stomach of the mud crab Macrophthalmus japonicus, an indicator species for assessing coastal benthic environments, when exposed to BPA or DEHP. After EDC exposure, the expression of lipopolysaccharide and β-1,3-glucan-binding protein (LGBP), a pattern recognition protein that activates the proPO system, was upregulated in the stomach of M. japonicus, whereas LGBP gene expression was downregulated in the gonad. In the gonad, which is a reproductive organ, EDC exposure mainly induced the transcriptional upregulation of trypsin-like serine protease (Tryp) at relatively low concentrations. In the stomach, which is a digestive organ, LGBP expression was upregulated at relatively low concentrations of EDCs over 7 days, whereas all proPO system-related genes (LGBP, Tryp, serine protease inhibitor (Serpin), and peroxinectin (PE)) responded to all concentrations of EDCs. These results suggest that the antioxidant and immune defense responses of the proPO system to EDC toxicity may vary, causing different degrees of damage depending on the tissue type in the mud crab.
Keywords
crustacean, immune defense system, mRNA expression, chemical pollutants, organ specificity
Subject
Environmental and Earth Sciences, Environmental Science
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
