Version 1
: Received: 17 September 2024 / Approved: 18 September 2024 / Online: 18 September 2024 (10:24:27 CEST)
How to cite:
Iribarnegaray, V.; Godiño, G.; Larrañaga, C.; Yamasaki, K.; Verdes, J. M.; Puentes, R. Droplet Digital Pcr Enhances Sensitivity and Accuracy in Canine Distemper Virus Detection. Preprints2024, 2024091390. https://doi.org/10.20944/preprints202409.1390.v1
Iribarnegaray, V.; Godiño, G.; Larrañaga, C.; Yamasaki, K.; Verdes, J. M.; Puentes, R. Droplet Digital Pcr Enhances Sensitivity and Accuracy in Canine Distemper Virus Detection. Preprints 2024, 2024091390. https://doi.org/10.20944/preprints202409.1390.v1
Iribarnegaray, V.; Godiño, G.; Larrañaga, C.; Yamasaki, K.; Verdes, J. M.; Puentes, R. Droplet Digital Pcr Enhances Sensitivity and Accuracy in Canine Distemper Virus Detection. Preprints2024, 2024091390. https://doi.org/10.20944/preprints202409.1390.v1
APA Style
Iribarnegaray, V., Godiño, G., Larrañaga, C., Yamasaki, K., Verdes, J. M., & Puentes, R. (2024). Droplet Digital Pcr Enhances Sensitivity and Accuracy in Canine Distemper Virus Detection. Preprints. https://doi.org/10.20944/preprints202409.1390.v1
Chicago/Turabian Style
Iribarnegaray, V., José Manuel Verdes and Rodrigo Puentes. 2024 "Droplet Digital Pcr Enhances Sensitivity and Accuracy in Canine Distemper Virus Detection" Preprints. https://doi.org/10.20944/preprints202409.1390.v1
Abstract
Canine distemper virus (CDV) poses a substantial threat to diverse carnivores, leading to sys-temic and often fatal diseases. Accurate and prompt diagnosis is paramount for effective man-agement and curbing further transmission. This study evaluates the diagnostic efficacy of drop-let digital PCR (ddPCR) in comparison to conventional reverse transcription (RT-PCR) and quantitative reverse transcription real-time PCR (RT-qPCR). Seventy-six clinical samples were collected from dogs with CDV symptoms diagnosed by specialized veterinarians, and sixteen samples from asymptomatic individuals. Conventional PCR, quantitative real-time PCR, and ddPCR were deployed, and their diagnostic capabilities were meticulously assessed. DdPCR ex-hibited heightened analytical sensitivity, reaching a detection limit of 3 copies/μL, whereas RT-qPCR had a detection limit of 86 copies/μL. The comparative analysis between clinical diagnosis and molecular techniques, including RT-PCR and RT-qPCR, demonstrated low concordance, with Kappa coefficients of 0.268 and 0.324, respectively. In contrast, ddPCR, showed a moderate concordance, with a Kappa coefficient of 0.477. Sensitivity was 42.39% for RT-PCR, 57.89% for RT-qPCR and 72.37% for ddPCR, with 100% of specificity for all methods. This study under-scores ddPCR's superior sensitivity and concordance with clinical CDV diagnosis, even at low viral concentrations, suggesting it as a promising alternative for CDV diagnosis.
Keywords
droplet Digital PCR; canine distemper virus; molecular diagnosis
Subject
Medicine and Pharmacology, Veterinary Medicine
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
