Isolation and Characterization of a Bacteriophage with Potential for the Control of Multidrug-Resistant Salmonella Strains Encoding Virulence Factors Associated with the Promotion of Precancerous Lesions

Luis Amarillas; Fedra Padilla; Rubén León-Chan; Jorge Padilla; Yadira Lugo-Melchor; Erique López-Avendaño; Luis Lightbourn; Mitzi Estrada-Acosta

doi:10.20944/preprints202409.1483.v1

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preprints.org > public health and healthcare > public health and health services > doi: 10.20944/preprints202409.1483.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Isolation and Characterization of a Bacteriophage with Potential for the Control of Multidrug-Resistant Salmonella Strains Encoding Virulence Factors Associated with the Promotion of Precancerous Lesions

Erique López-Avendaño

Luis Lightbourn

^* ,

Mitzi Estrada-Acosta

Version 1 : Received: 19 September 2024 / Approved: 19 September 2024 / Online: 19 September 2024 (10:41:10 CEST)

How to cite: Amarillas, L.; Padilla, F.; León-Chan, R.; Padilla, J.; Lugo-Melchor, Y.; López-Avendaño, E.; Lightbourn, L.; Estrada-Acosta, M. Isolation and Characterization of a Bacteriophage with Potential for the Control of Multidrug-Resistant Salmonella Strains Encoding Virulence Factors Associated with the Promotion of Precancerous Lesions. Preprints 2024, 2024091483. https://doi.org/10.20944/preprints202409.1483.v1 Amarillas, L.; Padilla, F.; León-Chan, R.; Padilla, J.; Lugo-Melchor, Y.; López-Avendaño, E.; Lightbourn, L.; Estrada-Acosta, M. Isolation and Characterization of a Bacteriophage with Potential for the Control of Multidrug-Resistant Salmonella Strains Encoding Virulence Factors Associated with the Promotion of Precancerous Lesions. Preprints 2024, 2024091483. https://doi.org/10.20944/preprints202409.1483.v1

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MDPI and ACS Style

Amarillas, L.; Padilla, F.; León-Chan, R.; Padilla, J.; Lugo-Melchor, Y.; López-Avendaño, E.; Lightbourn, L.; Estrada-Acosta, M. Isolation and Characterization of a Bacteriophage with Potential for the Control of Multidrug-Resistant Salmonella Strains Encoding Virulence Factors Associated with the Promotion of Precancerous Lesions. Preprints 2024, 2024091483. https://doi.org/10.20944/preprints202409.1483.v1

APA Style

Amarillas, L., Padilla, F., León-Chan, R., Padilla, J., Lugo-Melchor, Y., López-Avendaño, E., Lightbourn, L., & Estrada-Acosta, M. (2024). Isolation and Characterization of a Bacteriophage with Potential for the Control of Multidrug-Resistant <em>Salmonella</em> Strains Encoding Virulence Factors Associated with the Promotion of Precancerous Lesions. Preprints. https://doi.org/10.20944/preprints202409.1483.v1

Chicago/Turabian Style

Amarillas, L., Luis Lightbourn and Mitzi Estrada-Acosta. 2024 "Isolation and Characterization of a Bacteriophage with Potential for the Control of Multidrug-Resistant <em>Salmonella</em> Strains Encoding Virulence Factors Associated with the Promotion of Precancerous Lesions" Preprints. https://doi.org/10.20944/preprints202409.1483.v1

Abstract

Antimicrobial-resistant bacteria represent a serious threat to public health. Among these bacteria, Salmonella is of high priority because of its morbidity levels and its ability to induce different types of cancer. Aim: This study aimed to identify Salmonella strains encoding genes linked to the promotion of precancerous lesions and to isolate a bacteriophage to evaluating its preclinical potential against these bacteria. Methodology: An epidemiological approach based on wastewater analysis was employed to isolate Salmonella strains and detect genes associated with the induction of precancerous lesions. Antimicrobial susceptibility was assessed by disk diffusion method. A bacteriophage was isolated via the double agar technique, and its morphological characteristics, stability, host range, replication dynamics and ability to control Salmonella under different conditions were evaluated. The bacteriophage genome was sequenced and analyzed using bioinformatics tools. Results: Thirty-seven Salmonella strains were isolated, 17 of which contained the five genes associated with precancerous lesions induction. These strains exhibited resistance to multiple antimicrobials, including fluoroquinolones. A bacteriophage from the Au-tographiviridae family with lytic activity against 21 bacterial strains was isolated. This phage ex-hibited a 20 min replication cycle, releasing 52 ± 3 virions per infected cell. It demonstrated sta-bility and efficacy in reducing Salmonella concentration in simulated gastrointestinal conditions, and its genome lacked genes that represent a biosafety risk. Conclusion: This bacteriophage shows promising preclinical potential as a biotherapeutic agent against Salmonella.

Keywords

Antibiotic resistance; Wastewater epidemiology; Oncogenic Salmonella; Lytic phage

Subject

Public Health and Healthcare, Public Health and Health Services

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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