Recent Advancements In Hplc Method Development And Validation: Pushing The Boundaries Of Analysis

Shreya Talreja; Shashank Tiwari

doi:10.20944/preprints202409.1804.v1

Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 20 September 2024 / Approved: 23 September 2024 / Online: 23 September 2024 (15:04:55 CEST)

The high performance of liquid chromatography(HPLC) has long been an indispensable tool for vigilance and analysis across range disciplines such as pharmaceuticals, environmental monitoring, food safety attributes etc. We will discuss about HPLC method development and validation, types of liquid chromatography methods focusing on proper use of a mobile phase like an eluent selection, how to optimize the conditions as well take it through its various procedures required for approval from regulatory bodies. Strategies to improve method robustness, sensitivity and reproducibility are highlighted. The future breakthroughs in HPLC technology and some innovative trends have also been speculated to limit this fundamental methodology.

Method Development; Method Optimization; Chromatographic Conditions; Mobile Phase; Stationary Phase; Gradient Elution; Isocratic Elution; Sample Preparation; Detection Methods; UV-Vis Detection

Chemistry and Materials Science, Analytical Chemistry

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Recent Advancements In Hplc Method Development And Validation: Pushing The Boundaries Of Analysis

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