PreprintArticleVersion 1This version is not peer-reviewed
The Extract of Larrea tridentata Promotes the Synthesis of Silver Nanoparticles and Stimulates Immune Responses in Penaeus vannamei against Vibrio spp, Causing Acute Hepatopancreatic Necrosis Disease
Version 1
: Received: 26 September 2024 / Approved: 27 September 2024 / Online: 27 September 2024 (13:44:06 CEST)
How to cite:
León-Valdez, G.; Valenzuela-Quiñonez, W.; Álvarez-Ruiz, P.; Soto-Robles, C. A.; Nava-Perez, E.; López-Cervantes, G.; Montoya-Mejía, M. The Extract of Larrea tridentata Promotes the Synthesis of Silver Nanoparticles and Stimulates Immune Responses in Penaeus vannamei against Vibrio spp, Causing Acute Hepatopancreatic Necrosis Disease. Preprints2024, 2024092225. https://doi.org/10.20944/preprints202409.2225.v1
León-Valdez, G.; Valenzuela-Quiñonez, W.; Álvarez-Ruiz, P.; Soto-Robles, C. A.; Nava-Perez, E.; López-Cervantes, G.; Montoya-Mejía, M. The Extract of Larrea tridentata Promotes the Synthesis of Silver Nanoparticles and Stimulates Immune Responses in Penaeus vannamei against Vibrio spp, Causing Acute Hepatopancreatic Necrosis Disease. Preprints 2024, 2024092225. https://doi.org/10.20944/preprints202409.2225.v1
León-Valdez, G.; Valenzuela-Quiñonez, W.; Álvarez-Ruiz, P.; Soto-Robles, C. A.; Nava-Perez, E.; López-Cervantes, G.; Montoya-Mejía, M. The Extract of Larrea tridentata Promotes the Synthesis of Silver Nanoparticles and Stimulates Immune Responses in Penaeus vannamei against Vibrio spp, Causing Acute Hepatopancreatic Necrosis Disease. Preprints2024, 2024092225. https://doi.org/10.20944/preprints202409.2225.v1
APA Style
León-Valdez, G., Valenzuela-Quiñonez, W., Álvarez-Ruiz, P., Soto-Robles, C. A., Nava-Perez, E., López-Cervantes, G., & Montoya-Mejía, M. (2024). The Extract of <em>Larrea tridentata</em> Promotes the Synthesis of Silver Nanoparticles and Stimulates Immune Responses in <em>Penaeus vannamei</em> against <em>Vibrio</em> spp, Causing Acute Hepatopancreatic Necrosis Disease. Preprints. https://doi.org/10.20944/preprints202409.2225.v1
Chicago/Turabian Style
León-Valdez, G., Gabriela López-Cervantes and Magnolia Montoya-Mejía. 2024 "The Extract of <em>Larrea tridentata</em> Promotes the Synthesis of Silver Nanoparticles and Stimulates Immune Responses in <em>Penaeus vannamei</em> against <em>Vibrio</em> spp, Causing Acute Hepatopancreatic Necrosis Disease" Preprints. https://doi.org/10.20944/preprints202409.2225.v1
Abstract
Specific strains of Vibrio parahaemolyticus can cause acute hepatopancreatic necrosis disease (AHPND), a critical issue in shrimp aquaculture despite the application of several control strate-gies. The use of antibiotics is now restricted due to increasing bacterial resistance and overuse. Silver nanoparticles (AgNPs) have shown potential in shrimp aquaculture, with applications in boosting immunity against certain types of pathogens, promoting growth, and improving sur-vival rates. However, an economically viable solution that protect the organisms has not been found, which is why the search for nanoparticles synthesized with plant extracts is necessary to generate environmentally friendly control strategies. In this study, we synthesized AgNPs from Larrea tridentata extract and administered them orally with feed over a 35-day period. Shrimps fed with AgNPs-enriched diets showed a significant increase (p < 0.05) in mRNA expression of immune-related genes (CTL-5, MNK, SR, and GILT), particularly within the first 24–48 hours. No significant differences were observed in growth rates, but survival rates in a challenge against V. parahaemolyticus exceeded 85%, higher than the control group. Based on our findings and previ-ous literature, L. tridentata can effectively promote the synthesis of AgNPs and shows potential as an antimicrobial agent, without affecting the growth or survival of treated shrimp.
Keywords
Pacific white shrimp; Vibrio; green synthesis; AHPND; MIC
Subject
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
