Preprint Article Version 1 This version is not peer-reviewed

Chromosome-Level Genome Assembly and Annotation of the Crested Gecko, Correlophus ciliatus, a Lizard Incapable of Tail Regeneration

Version 1 : Received: 26 September 2024 / Approved: 27 September 2024 / Online: 30 September 2024 (11:04:57 CEST)

How to cite: Gumangan, M. A.; Pan, Z.; Lozito, T. Chromosome-Level Genome Assembly and Annotation of the Crested Gecko, Correlophus ciliatus, a Lizard Incapable of Tail Regeneration. Preprints 2024, 2024092389. https://doi.org/10.20944/preprints202409.2389.v1 Gumangan, M. A.; Pan, Z.; Lozito, T. Chromosome-Level Genome Assembly and Annotation of the Crested Gecko, Correlophus ciliatus, a Lizard Incapable of Tail Regeneration. Preprints 2024, 2024092389. https://doi.org/10.20944/preprints202409.2389.v1

Abstract

The vast majority of gecko species are capable of tail regeneration, but singular geckos of Correlophus, Uroplatus, and Nephrurus genera are unable to regrow lost tails. Of these non-regenerative geckos, the crested gecko (Correlophus ciliatus) is distinguished by ready availability, ease of care, high productivity, and hybridization potential. These features make C. ciliatus particularly suited as a model for studying the genetic, molecular, and cellular mechanisms underlying loss of tail regeneration capabilities. We report a contiguous genome of C. ciliatus with a total size of 1.65 Gb, a total of 152 scaffolds, L50 of 6, and N50 of 109 Mb. Repetitive content consists of 40.41% of the genome, and a total of 30,780 genes were annotated. Assembly of the crested gecko genome provides a valuable resource for future comparative genomic studies between non-regenerative and regenerative geckos and other squamate reptiles.

Keywords

Gekkota; crested gecko; Correlophus ciliatus; genome sequencing; assembly

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

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