Version 1
: Received: 30 September 2024 / Approved: 1 October 2024 / Online: 1 October 2024 (04:57:43 CEST)
How to cite:
Fernandes, T. L.; Santanna, J. P. C.; de Faria, R. R.; Pastore, E. R.; Bueno, D. F.; Hernandez, A. J. Tissue Engineering Construct for Articular Cartilage Restoration with Stromal Cells from Synovium vs Dental Pulp - A Pre-Clinical Study. Preprints2024, 2024100013. https://doi.org/10.20944/preprints202410.0013.v1
Fernandes, T. L.; Santanna, J. P. C.; de Faria, R. R.; Pastore, E. R.; Bueno, D. F.; Hernandez, A. J. Tissue Engineering Construct for Articular Cartilage Restoration with Stromal Cells from Synovium vs Dental Pulp - A Pre-Clinical Study. Preprints 2024, 2024100013. https://doi.org/10.20944/preprints202410.0013.v1
Fernandes, T. L.; Santanna, J. P. C.; de Faria, R. R.; Pastore, E. R.; Bueno, D. F.; Hernandez, A. J. Tissue Engineering Construct for Articular Cartilage Restoration with Stromal Cells from Synovium vs Dental Pulp - A Pre-Clinical Study. Preprints2024, 2024100013. https://doi.org/10.20944/preprints202410.0013.v1
APA Style
Fernandes, T. L., Santanna, J. P. C., de Faria, R. R., Pastore, E. R., Bueno, D. F., & Hernandez, A. J. (2024). Tissue Engineering Construct for Articular Cartilage Restoration with Stromal Cells from Synovium vs Dental Pulp - A Pre-Clinical Study. Preprints. https://doi.org/10.20944/preprints202410.0013.v1
Chicago/Turabian Style
Fernandes, T. L., Daniela Franco Bueno and Arnaldo José Hernandez. 2024 "Tissue Engineering Construct for Articular Cartilage Restoration with Stromal Cells from Synovium vs Dental Pulp - A Pre-Clinical Study" Preprints. https://doi.org/10.20944/preprints202410.0013.v1
Abstract
Introduction: Cartilage injuries and osteoarthritis are very prevalent and considered a public health problem, as they are highly disabling and represent an economic burden. Mesenchymal Stromal Cells (MSCs) can be isolated from different tissues and have the immunomodulatory capacity to regulate local articular joint environment. This translational study aims to compare the cartilage restoration from MSCs from the synovial membrane (SM) and dental pulp (DP), by tissue engineered treatment in a Good Manufacturing Practices.
Materials and Methods: A controlled experimental study in fourteen miniature pigs was performed, using scaffold-free Tissue Engineering Construct (TEC) from DP and SM MSCs, with 6 months follow-up. Total thickness cartilage defects were performed in both posterior knees. The defect was left empty on one side, and the other received TEC from DP (n=7) or from SM (n=7). MRI assessed morphology with the MOCART scoring system. T2 mapping assessed water and collagen fiber composition. Histology evaluated cartilage repair using the ICRS-2 score.
Results: The mean MOCART value in the untreated group was 46.2 ± 13.4, while the group treated with TEC from SM was 65.7 ± 15.5 (p
Medicine and Pharmacology, Orthopedics and Sports Medicine
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
