Preprint Article Version 1 This version is not peer-reviewed

Pathologic In Vivo Assay of the Helicobacter Molecular Infection in Stomach Tissue Using Carbon Nanotube Micro Sensor

Version 1 : Received: 3 October 2024 / Approved: 4 October 2024 / Online: 4 October 2024 (10:36:19 CEST)

How to cite: Lee, K.; Jun, S.; Oh, Y.; Ly, S. Y. Pathologic In Vivo Assay of the Helicobacter Molecular Infection in Stomach Tissue Using Carbon Nanotube Micro Sensor. Preprints 2024, 2024100300. https://doi.org/10.20944/preprints202410.0300.v1 Lee, K.; Jun, S.; Oh, Y.; Ly, S. Y. Pathologic In Vivo Assay of the Helicobacter Molecular Infection in Stomach Tissue Using Carbon Nanotube Micro Sensor. Preprints 2024, 2024100300. https://doi.org/10.20944/preprints202410.0300.v1

Abstract

Biomolecular detections of Helicobacter pylori(HP) were compared using specially modified sensors and fluorine immobilized on a carbon nanotube (HFCNT) electrode, which yielded sensitive results. Handheld voltammetric circuits were used for optimization. An anodic voltammogram of HP molecular oxidation was obtained at 0.0 V (versus the Ag/AgCl/KCl) in a 0.1M NH4H2PO4 electrolyte solution. Under optimized conditions, the analytical working range was 2.98x103-22.127x103 CFU/ml HP using square wave (SW) stripping voltammetry, precision of R2 =0.9857 (SWSV), the detection limit approached to 2.5x102 CFU/ml HP (S/N=3).The developed techniques have been applied to diagnosis of early-stage HP infections using stomach tissue from healthy humans and gastric patients.

Keywords

Voltammetry; stripping; helicobacter pylori; gastric patients; carbon nanotube micro sensor

Subject

Biology and Life Sciences, Behavioral Sciences

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