Preprint Article Version 1 This version is not peer-reviewed

The Presence of Some Minor Aspergillus and Penicillium Mycotoxins: Mycophenolic Acid, Cyclopiazonic Acid, Penicillic Acid, Roquefortine C, and Gliotoxin in Main Cereals Cultivated in Albania

Version 1 : Received: 5 October 2024 / Approved: 5 October 2024 / Online: 7 October 2024 (11:23:39 CEST)

How to cite: Topi, D.; Damani, Z.; Babič, J.; Jakovac-Strajn, B.; Tavčar-Kalcher, G. The Presence of Some Minor Aspergillus and Penicillium Mycotoxins: Mycophenolic Acid, Cyclopiazonic Acid, Penicillic Acid, Roquefortine C, and Gliotoxin in Main Cereals Cultivated in Albania. Preprints 2024, 2024100387. https://doi.org/10.20944/preprints202410.0387.v1 Topi, D.; Damani, Z.; Babič, J.; Jakovac-Strajn, B.; Tavčar-Kalcher, G. The Presence of Some Minor Aspergillus and Penicillium Mycotoxins: Mycophenolic Acid, Cyclopiazonic Acid, Penicillic Acid, Roquefortine C, and Gliotoxin in Main Cereals Cultivated in Albania. Preprints 2024, 2024100387. https://doi.org/10.20944/preprints202410.0387.v1

Abstract

(1) Background: Food and Feed Safety Legislation does not concern all the mycotoxins generated by Penicillium and Aspergillus spp. Certain mycotoxins, including cyclopiazonic acid (CPA), penicillic acid (PA), roquefortine C (ROQ C), mycophenolic acid (MPA), and gliotoxin regarded as having lower toxicity levels, hence not included in food and feed legislation; (2) Methods: Presence of these substances in maize and wheat grown in Albania across two consecutive harvesting seasons was investigated by liquid chromatography and mass spectrometry (LC-MS/MS); (3) Results: The findings indicated the presence of these mycotoxins in maize grain but not in wheat grain. In the 2014 season, they exhibited a higher contamination incidence than the 2015 season. The most commonly detected Mycotoxin was MPA, followed by CPA and ROC toxin, while Penicillium acid and gliotoxin were detected. The MPA revealed a concentration range of 72.92–3447.25 μg/kg, with a mean value of 1063.89 μg/kg. Mycophenolic acid was detected in the maize samples collected during the 2015 season. (4) Conclusions: These findings suggest that focusing the investigation only on 'controlled' mycotoxins will not produce a proper risk assessment and may not adequately address the possible harmful impacts of mycotoxins on human and animal health due to mycotoxins co-occurrence.

Keywords

Mycophenolic acid; Cyclopiazonic acid; Roquefortine C; Penicillic acid; gliotoxin; maize; wheat; LC-MS/MS; Albania

Subject

Chemistry and Materials Science, Analytical Chemistry

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