Version 1
: Received: 8 October 2024 / Approved: 9 October 2024 / Online: 9 October 2024 (10:36:56 CEST)
How to cite:
Qurbani, K.; Hussein, S.; Ahmed, S. K. Molecular Biotechniques for the Isolation and Identification of Microorganisms. Preprints2024, 2024100681. https://doi.org/10.20944/preprints202410.0681.v1
Qurbani, K.; Hussein, S.; Ahmed, S. K. Molecular Biotechniques for the Isolation and Identification of Microorganisms. Preprints 2024, 2024100681. https://doi.org/10.20944/preprints202410.0681.v1
Qurbani, K.; Hussein, S.; Ahmed, S. K. Molecular Biotechniques for the Isolation and Identification of Microorganisms. Preprints2024, 2024100681. https://doi.org/10.20944/preprints202410.0681.v1
APA Style
Qurbani, K., Hussein, S., & Ahmed, S. K. (2024). Molecular Biotechniques for the Isolation and Identification of Microorganisms. Preprints. https://doi.org/10.20944/preprints202410.0681.v1
Chicago/Turabian Style
Qurbani, K., Safin Hussein and Sirwan Khalid Ahmed. 2024 "Molecular Biotechniques for the Isolation and Identification of Microorganisms" Preprints. https://doi.org/10.20944/preprints202410.0681.v1
Abstract
Microorganisms play a pivotal role in ecology, medicine, agriculture, and biotechnology. To comprehend their diversity, physiology, and functions, isolation and characterization are imperative. This review provides a concise overview of prevalent molecular biotechniques for microbial isolation. It describes the advantages, constraints, and practical applications of both culture-dependent and culture-independent approaches. Discussed methods encompass selective/differential media, 16S rRNA gene sequencing, metagenomics, fluorescence in situ hybridization (FISH), polymerase chain reaction (PCR)-based techniques, and single-cell genomics. Emerging high-throughput sequencing and single-cell isolation methods exemplify cutting-edge procedures explored herein. Integration of these molecular tools offers comprehensive insights into microbial-diversity, community structure, metabolic capabilities, and functional interplays.
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
