PreprintArticleVersion 1This version is not peer-reviewed
One Health Approach to Study the Occurrence and Antimicrobial Resistance of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli and Klebsiella spp. in Urban Agriculture in Burkina Faso
Version 1
: Received: 8 October 2024 / Approved: 8 October 2024 / Online: 9 October 2024 (14:44:56 CEST)
How to cite:
Diarra, F. B. J.; Bonkoungou, I. J. O.; Garba, Z.; Somda, N. S.; Soma, D.; Nikiema, M. E. M.; Bako, E.; Sawadogo, N.; Barro, N.; Haukka, K. One Health Approach to Study the Occurrence and Antimicrobial Resistance of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli and Klebsiella spp. in Urban Agriculture in Burkina Faso. Preprints2024, 2024100693. https://doi.org/10.20944/preprints202410.0693.v1
Diarra, F. B. J.; Bonkoungou, I. J. O.; Garba, Z.; Somda, N. S.; Soma, D.; Nikiema, M. E. M.; Bako, E.; Sawadogo, N.; Barro, N.; Haukka, K. One Health Approach to Study the Occurrence and Antimicrobial Resistance of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli and Klebsiella spp. in Urban Agriculture in Burkina Faso. Preprints 2024, 2024100693. https://doi.org/10.20944/preprints202410.0693.v1
Diarra, F. B. J.; Bonkoungou, I. J. O.; Garba, Z.; Somda, N. S.; Soma, D.; Nikiema, M. E. M.; Bako, E.; Sawadogo, N.; Barro, N.; Haukka, K. One Health Approach to Study the Occurrence and Antimicrobial Resistance of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli and Klebsiella spp. in Urban Agriculture in Burkina Faso. Preprints2024, 2024100693. https://doi.org/10.20944/preprints202410.0693.v1
APA Style
Diarra, F. B. J., Bonkoungou, I. J. O., Garba, Z., Somda, N. S., Soma, D., Nikiema, M. E. M., Bako, E., Sawadogo, N., Barro, N., & Haukka, K. (2024). One Health Approach to Study the Occurrence and Antimicrobial Resistance of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli and Klebsiella spp. in Urban Agriculture in Burkina Faso. Preprints. https://doi.org/10.20944/preprints202410.0693.v1
Chicago/Turabian Style
Diarra, F. B. J., Nicolas Barro and Kaisa Haukka. 2024 "One Health Approach to Study the Occurrence and Antimicrobial Resistance of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli and Klebsiella spp. in Urban Agriculture in Burkina Faso" Preprints. https://doi.org/10.20944/preprints202410.0693.v1
Abstract
Data on antimicrobial resistance in Burkina Faso’s agricultural sector is still limited. The study assessed the occurrence of extended spectrum β-lactamase -producing Escherichia coli (ESBL-Ec) and Klebsiella spp. (ESBL-K) in lettuce, environment, and gardeners’ stools in market gardens in Ouagadougou, Burkina Faso. A total of 356 samples were collected from three vegetable gardens (76 lettuce, 76 soil, 62 manure, 63 irrigation water and 79 human stools). ESBL-selective medium was used for initial selection of ESBL-producing bacteria, and the isolates were further identified using biochemical tests. Antibiotic susceptibility test was performed using the disk diffusion method. The overall prevalence of ESBL-Ec and/or ESBL-K in the samples was 232/356 (65.2%). Of the lettuce samples, the prevalence of ESBL-Ec was 19/76 (25.0%) and ESBL-K 33/76 (43.4%). In the market gardens environment, the prevalence of ESBL-Ec was 32/201 (15.9%) and ESBL-K 124/201 (61.7%). In the gardeners’ stools the prevalence of ESBL-Ec was 42/79 (53.2%) and ESBL-K 24/79 (30.4%). Two ESBL-K isolates were found to produce NDM carbapenemase. Due to the high prevalence of ESBL-producing bacteria, which may also be carbapenemase producers, it is necessary to monitor pathogens in agricultural products with a "One Health" approach to limit and prevent infections in the population.
Public Health and Healthcare, Public Health and Health Services
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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