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Virtual Screening and Meta-Analysis Approach Identifies Factors for Inversion Stimulation (Fis) and Other Genes Responsible for Biofilm Production in Pseudomonas aeruginosa: A Corneal Pathogen
Version 1
: Received: 14 October 2024 / Approved: 15 October 2024 / Online: 15 October 2024 (04:24:46 CEST)
How to cite:
Emeka, P. M.; Badger-Emeka, L. I.; Thirugnanasambantham, K. Virtual Screening and Meta-Analysis Approach Identifies Factors for Inversion Stimulation (Fis) and Other Genes Responsible for Biofilm Production in Pseudomonas aeruginosa: A Corneal Pathogen. Preprints2024, 2024101121. https://doi.org/10.20944/preprints202410.1121.v1
Emeka, P. M.; Badger-Emeka, L. I.; Thirugnanasambantham, K. Virtual Screening and Meta-Analysis Approach Identifies Factors for Inversion Stimulation (Fis) and Other Genes Responsible for Biofilm Production in Pseudomonas aeruginosa: A Corneal Pathogen. Preprints 2024, 2024101121. https://doi.org/10.20944/preprints202410.1121.v1
Emeka, P. M.; Badger-Emeka, L. I.; Thirugnanasambantham, K. Virtual Screening and Meta-Analysis Approach Identifies Factors for Inversion Stimulation (Fis) and Other Genes Responsible for Biofilm Production in Pseudomonas aeruginosa: A Corneal Pathogen. Preprints2024, 2024101121. https://doi.org/10.20944/preprints202410.1121.v1
APA Style
Emeka, P. M., Badger-Emeka, L. I., & Thirugnanasambantham, K. (2024). Virtual Screening and Meta-Analysis Approach Identifies Factors for Inversion Stimulation (Fis) and Other Genes Responsible for Biofilm Production in <em>Pseudomonas aeruginosa</em>: A Corneal Pathogen. Preprints. https://doi.org/10.20944/preprints202410.1121.v1
Chicago/Turabian Style
Emeka, P. M., Lorina Ineta Badger-Emeka and Krishnaraj Thirugnanasambantham. 2024 "Virtual Screening and Meta-Analysis Approach Identifies Factors for Inversion Stimulation (Fis) and Other Genes Responsible for Biofilm Production in <em>Pseudomonas aeruginosa</em>: A Corneal Pathogen" Preprints. https://doi.org/10.20944/preprints202410.1121.v1
Abstract
Bacterial keratitis caused by Pseudomonas aeruginosa is indeed a serious concern due to its potential to cause blindness and its resistance to antibiotics, partly attributed to biofilm formation and cytotoxicity to the cornea. The present study uses meta-analysis of transcriptomics dataset to identify important genes and pathways in biofilm formation of P. aeruginosa induced keratitis. By combining data from several studies, meta-analysis can enhance statistical power and robustness, enabling the identification of 83 differentially expressed candidate genes including fis that could serve as therapeutic targets. The approach of combining meta-analysis with virtual screening and in vitro methods provides a comprehensive strategy for identifying potential target genes and pathway crucial for bacterial biofilm formation and development anti-biofilm medications against P. aeruginosa infections. The study identified 83 candidate genes that exhibited differential expression in the biofilm state, with fis proposed as an ideal target for therapy for P. aeruginosa biofilm formation. These techniques, meta-analysis, virtual screening, and in-vitro methods were used in combination to diagnostically identify these genes which play a significant role in biofilms. This finding has highlighted a hallmark target list for P. aeruginosa anti-biofilm potential treatments.
Medicine and Pharmacology, Medicine and Pharmacology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
