PreprintArticleVersion 1This version is not peer-reviewed
Exploring the Potential of Anastatica hierochuntica-Integrated Nanofibers as a Promising Nanoplatform for Enhanced Biofilm Disruption and Accelerated wound Healing
Version 1
: Received: 15 October 2024 / Approved: 15 October 2024 / Online: 15 October 2024 (11:30:25 CEST)
How to cite:
Abdelhakeem, E.; Hashem, M. M.; Attia, H.; Abdel Khalek, M. A.; M. Badr-Eldin, S.; M. Adel, I. Exploring the Potential of Anastatica hierochuntica-Integrated Nanofibers as a Promising Nanoplatform for Enhanced Biofilm Disruption and Accelerated wound Healing. Preprints2024, 2024101181. https://doi.org/10.20944/preprints202410.1181.v1
Abdelhakeem, E.; Hashem, M. M.; Attia, H.; Abdel Khalek, M. A.; M. Badr-Eldin, S.; M. Adel, I. Exploring the Potential of Anastatica hierochuntica-Integrated Nanofibers as a Promising Nanoplatform for Enhanced Biofilm Disruption and Accelerated wound Healing. Preprints 2024, 2024101181. https://doi.org/10.20944/preprints202410.1181.v1
Abdelhakeem, E.; Hashem, M. M.; Attia, H.; Abdel Khalek, M. A.; M. Badr-Eldin, S.; M. Adel, I. Exploring the Potential of Anastatica hierochuntica-Integrated Nanofibers as a Promising Nanoplatform for Enhanced Biofilm Disruption and Accelerated wound Healing. Preprints2024, 2024101181. https://doi.org/10.20944/preprints202410.1181.v1
APA Style
Abdelhakeem, E., Hashem, M. M., Attia, H., Abdel Khalek, M. A., M. Badr-Eldin, S., & M. Adel, I. (2024). Exploring the Potential of Anastatica hierochuntica-Integrated Nanofibers as a Promising Nanoplatform for Enhanced Biofilm Disruption and Accelerated wound Healing. Preprints. https://doi.org/10.20944/preprints202410.1181.v1
Chicago/Turabian Style
Abdelhakeem, E., Shaimaa M. Badr-Eldin and Islam M. Adel. 2024 "Exploring the Potential of Anastatica hierochuntica-Integrated Nanofibers as a Promising Nanoplatform for Enhanced Biofilm Disruption and Accelerated wound Healing" Preprints. https://doi.org/10.20944/preprints202410.1181.v1
Abstract
Natural components are sparking a lot of interest in the field of drug formulation and delivery. Anastatica hierochuntica is a small plant, extremely rich in its phytochemical content including flavonoids, phenolics, and several minerals. For that, it is widely used in folk medicine in a plethora of conditions such as headache, asthma, menstrual pain, and childbirth. The aim of this research is to investigate its potential antimicrobial effects when loaded onto nanofiber as field leading. The extract-loaded nanofibers were prepared via electrospinning. The prepared nanofibers were rod-shaped with nanometric size as revealed by scanning electron microscopy. The loaded NFs showed increased hydrophilicity compared to the plain NFs as evidenced by reduced WCA and increased percent water uptake. Further, they exhibited adequate tensile properties compared to the plain NFs as evidenced by increased strain and stress. Based on the highest hydrophilicity (WCA 43.1±0.6º and % swelling 216.67±2.36% after 1 h) and the best mechanical properties (strain and stress of 67.6% and 0.0486 N/mm2, respectively), NF20 was selected for further investigations. The selected formulation (NF20) showed a bi-phasic, sustained drug release pattern (73.40±1.31% after 72 h). More importantly, testing the antimicrobial effects of the extract-loaded nanofibers highlighted significant reduction in MIC, MBC, and MFC values when compared with Silymarin standard against Acinetobacter baumannii, Pseudomonas aeruginosa, Staphylococcus aureus, and Aspergillus niger. Finally, wound healing assay confirmed the accelerated wound closure and fibroblasts migration in case of the fabricated NF20, suggesting the potential of the bio- fabricated nanofiber as a novel promising wound dressing.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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