preprints.org > chemistry and materials science > organic chemistry > doi: 10.20944/preprints202410.1986.v1

Preprint Article Version 1 This version is not peer-reviewed

Version 1 : Received: 24 October 2024 / Approved: 24 October 2024 / Online: 25 October 2024 (11:43:16 CEST)

Plants of the subfamily Amaryllidoideae are a source of unique and bioactive alkaloids called Amaryllidaceae alkaloids. The study of their anticancer potential has intensified in recent years. This work aims to locate and characterize the profile of cytotoxic alkaloids biosynthesized and stored in different tissues of Phaedranassa lehmannii Regel using different histochemical methods and chromatographic analysis. The histochemical analysis in the bulbs revealed the presence of alkaloids at the basal edge of scale-like leaves and bud apical zone. The GC-MS analysis indicated that the bulbs biosynthesize alkaloids distributed in crinine- (9.80 µg/g DW), galanthamine- (8.04 µg/g DW), lycorine- (7.38 µg/g DW) and narciclasine-type (3.75 µg/g DW). The root biosynthesizes alkaloids distributed mostly in lycorine- (225.29 µg/g DW) and galanthamine-type (0.723 µg/g DW) alkaloids. The total alkaloids biosynthesized by the root (324.93 µg/g DW) exceeded eleven times the abundance of the alkaloids identified in the bulbs (28.97 µg/g DW). In addition, the total alkaloids fractions exhibited a cytotoxic effect dose‐dependent in the evaluated concentrations with IC50 values of 11.76 ± 0.99 µg/mL and 2.59 ± 0.56 µg/mL against human lung (A549) cancer cells and 8.00 ± 1.35 µg/mL and 18.74 ± 1.99 µg/mL against gastric (AGS) cancer cells. The present study provided evidence to locate and characterize the alkaloids of P. lehmannii grown under nursery conditions as a species producing potential antiproliferative alkaloids.

Phaedranassa lehmannii; Amaryllidaceae alkaloids; cytotoxicity; histochemical localization.

Chemistry and Materials Science, Organic Chemistry

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