Version 1
: Received: 28 October 2024 / Approved: 28 October 2024 / Online: 28 October 2024 (14:58:10 CET)
How to cite:
Fazolin Petrucelli, M.; Martins-Santana, L.; Oliveira, V. M.; Sanches, P. R.; Rossi, A.; Martinez-Rossi, N. M. The Transcription Factor StuA Regulates Oxidative Stress-Responsive Genes in Trichophyton rubrum. Preprints2024, 2024102192. https://doi.org/10.20944/preprints202410.2192.v1
Fazolin Petrucelli, M.; Martins-Santana, L.; Oliveira, V. M.; Sanches, P. R.; Rossi, A.; Martinez-Rossi, N. M. The Transcription Factor StuA Regulates Oxidative Stress-Responsive Genes in Trichophyton rubrum. Preprints 2024, 2024102192. https://doi.org/10.20944/preprints202410.2192.v1
Fazolin Petrucelli, M.; Martins-Santana, L.; Oliveira, V. M.; Sanches, P. R.; Rossi, A.; Martinez-Rossi, N. M. The Transcription Factor StuA Regulates Oxidative Stress-Responsive Genes in Trichophyton rubrum. Preprints2024, 2024102192. https://doi.org/10.20944/preprints202410.2192.v1
APA Style
Fazolin Petrucelli, M., Martins-Santana, L., Oliveira, V. M., Sanches, P. R., Rossi, A., & Martinez-Rossi, N. M. (2024). The Transcription Factor StuA Regulates Oxidative Stress-Responsive Genes in Trichophyton rubrum. Preprints. https://doi.org/10.20944/preprints202410.2192.v1
Chicago/Turabian Style
Fazolin Petrucelli, M., Antonio Rossi and Nilce M. Martinez-Rossi. 2024 "The Transcription Factor StuA Regulates Oxidative Stress-Responsive Genes in Trichophyton rubrum" Preprints. https://doi.org/10.20944/preprints202410.2192.v1
Abstract
Fungi can remarkably sense and adapt to various extracellular stimuli and stress conditions. Oxidative stress, which results from an imbalance between reactive oxygen species production and antioxidant defenses, leads to cellular damage and death. In Trichophyton rubrum, oxidative stress is managed by a complex antioxidant system including thioredoxins, glutathione, catalases, peroxidases, and superoxide dismutase, with glutathione playing a crucial role. The fungus also responds to oxidative stress through critical pathways such as the glycerol high osmolarity pathway, activator protein 1 transcription factor, and responsive regulator SKN7. To better understand the role of the transcription factor StuA in regulating oxidative stress-related genes within these pathways, we conducted gene expression studies in ΔstuA mutant and wild-type strains of T. rubrum cultivated in keratin and under oxidative stress induced by hydrogen peroxide. Our results revealed significant downregulation of essential antioxidant genes, including glutathione transferases and catalases, in the ΔstuA mutant. Moreover, catalase and glutathione S-transferase activities were impaired in the mutants under stress conditions, highlighting the impact of this mutation. These findings underscore the critical role of StuA in the oxidative stress response and fungal pathogenesis and provide new insights into T. rubrum's adaptive mechanisms.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
