Fan, K.; Feng, Z.; Zhao, D.; Guo, J.; Zheng, X.; Yin, W.; Sun, N.; Sun, P.; Li, H. Porcine Recombinant Nk-Lysin Inhibits the Growth and Metastasis of Hepatocellular Carcinoma In Vivo. Preprints2024, 2024102219. https://doi.org/10.20944/preprints202410.2219.v1
APA Style
Fan, K., Feng, Z., Zhao, D., Guo, J., Zheng, X., Yin, W., Sun, N., Sun, P., & Li, H. (2024). Porcine Recombinant Nk-Lysin Inhibits the Growth and Metastasis of Hepatocellular Carcinoma In Vivo. Preprints. https://doi.org/10.20944/preprints202410.2219.v1
Chicago/Turabian Style
Fan, K., Panpan Sun and Hongquan Li. 2024 "Porcine Recombinant Nk-Lysin Inhibits the Growth and Metastasis of Hepatocellular Carcinoma In Vivo" Preprints. https://doi.org/10.20944/preprints202410.2219.v1
Abstract
Porcine recombinant NK-lysin (prNK-lysin) has been shown to inhibit the proliferation and metastasis of hepatocellular carcinoma (HCC) cells in vitro. However, its effects on the proliferation and metastasis of HCC cells in vivo remain unclear. In this study, allograft murine model using the murine HCC cell line Hepa1-6 was employed to investigate the anticancer effects of prNK-lysin. Initially, the in vitro anticancer efficacy of prNK-lysin was evaluated on Hepa1-6 cells, demonstrating that prNK-lysin effectively inhibited both proliferation and metastasis through the induction of oncosis. Subsequently, the in vivo anticancer efficacy of prNK-lysin was assessed using a mouse liver orthotopic implantation model and a lung metastasis model of Hepa1-6 cells in BALB/cA-nu mice. Administration of 13 mg/kg prNK-lysin could inhibited tumor growth in the liver and metastasis to the lungs. Our results demonstrate that prNK-lysin possesses strong anti-HCC effects both in vitro and in vivo, with the induction of oncosis as potential molecular mechanisms for its anticancer activity.
Biology and Life Sciences, Biochemistry and Molecular Biology
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