Background: The present study investigated the effects of a micronutrient combination with proven impact on prostate cell metabolism and growth with regard to PSA dynamics. Methods: In an open single-arm multicenter study, 46 men with different baseline situations were recruited: 16 men with constantly elevated PSA values and negative prostate biopsy (group A), and 30 men with low risk prostate cancer on Active Surveillance (Group B) Results: In group A, 12 out of 16 men (75%) showed a decrease of PSA after 6 months from an average 9.26 to an average 8.84 ng/ml (4.5%), whereas in group B, PSA level decreased in 16, increased in 12 men, and remained unchanged in 1 man. The average PSA in group B dropped from 7.47 to 6.82 ng/ml (8.6%).Both PSA decreases were not statistically significant. Conclusion: The results of the present study are consistent with previous findings on the positive effects of individual micronutrients on prostate health. Longer administration of micronutrients or different dosage or combinations may further improve PSA outcomes.