Version 1
: Received: 4 November 2024 / Approved: 4 November 2024 / Online: 5 November 2024 (09:24:20 CET)
How to cite:
Makgobole, M. U.; Onwubu, S.; Baruwa, A.; Mpofana, N.; Obiechefu, Z.; Naidoo, D.; Khathi, A.; Mkhwanazi, B. N. Optimization of Collagen Extraction from Fish Scales Using Tris-Glycine Buffer: A Taguchi Methodological Approach. Preprints2024, 2024110220. https://doi.org/10.20944/preprints202411.0220.v1
Makgobole, M. U.; Onwubu, S.; Baruwa, A.; Mpofana, N.; Obiechefu, Z.; Naidoo, D.; Khathi, A.; Mkhwanazi, B. N. Optimization of Collagen Extraction from Fish Scales Using Tris-Glycine Buffer: A Taguchi Methodological Approach. Preprints 2024, 2024110220. https://doi.org/10.20944/preprints202411.0220.v1
Makgobole, M. U.; Onwubu, S.; Baruwa, A.; Mpofana, N.; Obiechefu, Z.; Naidoo, D.; Khathi, A.; Mkhwanazi, B. N. Optimization of Collagen Extraction from Fish Scales Using Tris-Glycine Buffer: A Taguchi Methodological Approach. Preprints2024, 2024110220. https://doi.org/10.20944/preprints202411.0220.v1
APA Style
Makgobole, M. U., Onwubu, S., Baruwa, A., Mpofana, N., Obiechefu, Z., Naidoo, D., Khathi, A., & Mkhwanazi, B. N. (2024). Optimization of Collagen Extraction from Fish Scales Using Tris-Glycine Buffer: A Taguchi Methodological Approach. Preprints. https://doi.org/10.20944/preprints202411.0220.v1
Chicago/Turabian Style
Makgobole, M. U., Andile Khathi and Blessing Nkazimulo Mkhwanazi. 2024 "Optimization of Collagen Extraction from Fish Scales Using Tris-Glycine Buffer: A Taguchi Methodological Approach" Preprints. https://doi.org/10.20944/preprints202411.0220.v1
Abstract
Collagen, a critical biomaterial with wide applications in pharmaceuticals, cosmetics, and medical industries, can be sourced sustainably from fish scales. This study optimizes the extraction of col-lagen using Tris-Glycine buffer from fish scales via the Taguchi method. Various extraction pa-rameters—buffer concentration, temperature, pH, and time—were evaluated to identify optimal conditions. Under optimal conditions (0.5 of acetic acids, volume of acids of 100mL, soaking time of 120 min, and Tris-Glycine buffer of 10mL), the results demonstrate that temperature and buffer concentration significantly influence collagen yield, with a collagen purity of 17.14 ± 0.05 mg/g. R2 value of 73.84% was obtained for the mathematical model). FTIR analysis confirmed the pres-ence of characteristic collagen peaks at 1611 cm⁻¹ (amide I), 1523 cm⁻¹ (amide II), and 1300 cm⁻¹ (amide III), indicating the successful extraction of Type I collagen. SDS-PAGE analysis revealed a protein banding pattern consistent with the molecular weight of collagen, and amino acid analysis shows high percentages of glycine (20.98%), proline (15.43%), and hydroxyproline (11.51%), im-plying fibrous collagen structures. The finding suggests that the Taguchi method offers an efficient and sustainable approach for collagen extraction, reducing waste from fish processing industries.
Keywords
Collagen extraction; fish scales; Tris-Glycine buffer; Taguchi method; sustainable biomaterials
Subject
Chemistry and Materials Science, Biomaterials
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
