Version 1
: Received: 1 November 2024 / Approved: 4 November 2024 / Online: 5 November 2024 (09:30:01 CET)
How to cite:
Sirekbasan, S.; Gürkök-Tan, T. Temporal Expression Dynamics of lncRNAs and Cis-Target Gene Interactions in Leishmania major-Infected Human Macrophages. Preprints2024, 2024110262. https://doi.org/10.20944/preprints202411.0262.v1
Sirekbasan, S.; Gürkök-Tan, T. Temporal Expression Dynamics of lncRNAs and Cis-Target Gene Interactions in Leishmania major-Infected Human Macrophages. Preprints 2024, 2024110262. https://doi.org/10.20944/preprints202411.0262.v1
Sirekbasan, S.; Gürkök-Tan, T. Temporal Expression Dynamics of lncRNAs and Cis-Target Gene Interactions in Leishmania major-Infected Human Macrophages. Preprints2024, 2024110262. https://doi.org/10.20944/preprints202411.0262.v1
APA Style
Sirekbasan, S., & Gürkök-Tan, T. (2024). Temporal Expression Dynamics of lncRNAs and Cis-Target Gene Interactions in Leishmania major-Infected Human Macrophages. Preprints. https://doi.org/10.20944/preprints202411.0262.v1
Chicago/Turabian Style
Sirekbasan, S. and Tuğba Gürkök-Tan. 2024 "Temporal Expression Dynamics of lncRNAs and Cis-Target Gene Interactions in Leishmania major-Infected Human Macrophages" Preprints. https://doi.org/10.20944/preprints202411.0262.v1
Abstract
Leishmaniasis is a serious infectious disease caused by Leishmania parasites, predominantly affecting tropical and subtropical regions. These parasites replicate within macrophages, manipulating the host immune response and facilitating infection progression. This study examined the expression profiles of long non-coding RNAs (lncRNAs), potential cis-target genes, and hub genes at different time points in human macrophages infected with Leishmania major. RNA-Seq analysis identified 39,828 lncRNAs, with 2,903 showing differential expression at one or more time points. As the infection progressed (4, 24, 48, and 72 hours), the number of up- and down-regulated lncRNAs gradually decreased. Six lncRNAs (lnc-UNC5D-8, lnc-TENM3-1, DIRC3-1, lnc-MTRNR2L12-10, lnc-FAM43A-6, and AKAP2-1) were consistently differentially expressed across all time points, potentially playing key roles in regulating the host immune response. Time-specific hub genes were also identified, regulating critical processes such as keratinization, epigenetic modifications, and immune responses. In particular, these genes were pivotal during the later stages of infection in maintaining tissue integrity and regulating immune responses. Early immune responses were dominated by immunoglobulin receptor activity and adaptive immune system activation. These findings highlight the critical roles of lncRNAs and hub genes in macrophage responses to Leishmania infection, offering potential targets for future therapeutic strategies.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
