Version 1
: Received: 6 November 2024 / Approved: 7 November 2024 / Online: 7 November 2024 (10:37:12 CET)
How to cite:
Seane, E.; Nair, S.; Vandevoorde, C.; Bisio, A.; Joubert, A. Cudc-101 and Proton Irradiation Reduces Migration and Invasion Capacity of Triple Negative Breast Cell Line. Preprints2024, 2024110513. https://doi.org/10.20944/preprints202411.0513.v1
Seane, E.; Nair, S.; Vandevoorde, C.; Bisio, A.; Joubert, A. Cudc-101 and Proton Irradiation Reduces Migration and Invasion Capacity of Triple Negative Breast Cell Line. Preprints 2024, 2024110513. https://doi.org/10.20944/preprints202411.0513.v1
Seane, E.; Nair, S.; Vandevoorde, C.; Bisio, A.; Joubert, A. Cudc-101 and Proton Irradiation Reduces Migration and Invasion Capacity of Triple Negative Breast Cell Line. Preprints2024, 2024110513. https://doi.org/10.20944/preprints202411.0513.v1
APA Style
Seane, E., Nair, S., Vandevoorde, C., Bisio, A., & Joubert, A. (2024). Cudc-101 and Proton Irradiation Reduces Migration and Invasion Capacity of Triple Negative Breast Cell Line. Preprints. https://doi.org/10.20944/preprints202411.0513.v1
Chicago/Turabian Style
Seane, E., Alessandra Bisio and Anna Joubert. 2024 "Cudc-101 and Proton Irradiation Reduces Migration and Invasion Capacity of Triple Negative Breast Cell Line" Preprints. https://doi.org/10.20944/preprints202411.0513.v1
Abstract
Radiation therapy remains to be one of the main treatment modalities for the management of breast cancer. Several reports have asserted that low dose photon irradiation, which is the most available radiation modality, can promote migration and invasion of malignant cells. Also, some approved chemotherapy drugs used in the management of breast cancer are implicated in promoting metastasis. Therefore, it has become critical to unravel novel therapies that are effective on tumour cells and can reduce the metastatic potential. Methods: Malignant (MCF-7), triple-negative (MDA-MB-231) and spontaneously immortalized MCF-10A human breast cancer cell lines were pre-treated with pan-HDACi SAHA or multi-target inhibitor CUDC-101 and exposed to 2Gy and 6Gy 148 MeV mid-SOBP protons or 250KeV X-rays. Wound healing and trans-well invasion assays were performed. Imaging was done at 4-hour intervals for wound healing assays, and at 24-hours post-irradiation for trans-well migration assays. Results: Significant reduction in migration was observed after treatment with proton alone at lower doses (2Gy) and at higher (6Gy) doses in the MCF-7 cell line. In the triple negative cell line MDA-MB-231 cell line, reduction in migration was evident at higher doses(6Gy) protons. Combination therapy of CUDC-101 or SAHA and protons also inhibited migration and invasion in the MDA-MB-231 cell line. Treatment with CUDC-101 monotherapy showed benefit in reducing migration and invasion in the MDA-MB-231 cell line. Treatment with SAHA, either as monotherapy or in combination with X-rays, promoted migration in the MCF-7 cell line. Conclusions: CUDC-101 or proton monotherapies, as well as combination therapy with radiation, inhibits migration as monotherapy particularly in the MDA-MB-231 which has high metastatic potential.
Keywords
metastasis; cell migration and invasion; Histone deacetylase inhibitor; triple negative breast cancer
Subject
Biology and Life Sciences, Biophysics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
